Crataegus sinaica defatted methanolic extract ameliorated monosodium iodoacetate-induced oxidative stress and inhibited inflammation in a rat model of osteoarthritis

Ifat A. Alsharif , Reham M. Abd-Elsalam, Mohammed S. Amer, Ahmed H. El-Desoky, Rehab F. Abdel-Rahman

Abstract


Background and purpose: Osteoarthritis is a degenerative joint disease without definite treatment. It is characterized by intra-articular inflammation, cartilage degeneration, subchondral bone remodeling, and joint pain. The objective of the current study was to assess the anti-osteoarthritic effect and the possible underlying mechanism of action of Crataegus sinaica extract (CSE).
Experimental approach: Intra-articular injection of monosodium iodoacetate in the right knee joint of all rats was done except for the sham group. One week later, the anti-inflammatory efficacy of CSE (100, 200, 300 mg/kg, daily p.o) for 4 successive weeks versus ibuprofen (40 mg/kg, p.o) was assessed.
Serum inflammatory cytokines; as well as weekly assessment of knee joint swelling, joint mobility, and motor coordination were done. At the end of the experiment, a histopathological investigation of the affected knee joints and an x-ray investigation were also executed.
Findings / Results: CSE significantly decreased joint swelling, pain behaviors, and serum levels of TNF-α, IL6, hyaluronic acid, and CTX-II. The radiographic findings revealed almost normal joint space with normal radiodensity and diameter in CSE-treated rats. As well, the histopathological and immunohistochemical investigations of the knee joints in CSE-treated groups retained the cartilage structure of knee joints. A significant reduction in the percentage of caspase-3-stained chondrocytes and a decrease in TGF-β1 immuno-positive areas in the synovial lining and sub lining were recorded in CSE-treated rats, compared to the osteoarthritis control group.
Conclusion and implications: This study approved the chondroprotective effects of CSE, and its ability to inhibit the pain associated with osteoarthritis.


Keywords


Crataegus sinaica; Inflammation; Monosodium iodoacetate; Osteoarthritis; Pain; Rats.

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References


Zhang W, Ouyang H, Dass CR, Xu J. Current research on pharmacologic and regenerative therapies for osteoarthritis. Bone Res. 2016;4:15040,1-14. DOI: 10.1038/boneres.2015.40.

Ma Y, Guo H, Bai F, Zhang M, Yang L, Deng J, et al. A rat model of knee osteoarthritis suitable for electroacupuncture study. Exp Anim. 2018;67(2):271-280. DOI: 10.1538/expanim.17-0142.

Oo WM, Little C, Duong V, Hunter DJ. The Development of disease-modifying therapies for osteoarthritis (DMOADs): the evidence to date. Drug Des Devel Ther. 2021;15:2921-2945. DOI: 10.2147/DDDT.S295224.

Abo-zalam HB, Abdelsalam RM, Abdel-Rahman RF, Abd-Ellah MF, Khattab MM. In vivo investigation of the ameliorating effect of tempol against MIA-induced knee osteoarthritis in rats: involvement of TGF-β1/SMAD3/NOX4 Cue. Molecules. 2021;26(22):6993,1-20. DOI: 10.3390/molecules26226993.

Nguyen QTN, Fang M, Zhang M, Do NQ, Kim M, Zheng SD, et al. Crataegus laevigata suppresses LPS-induced oxidative stress during inflammatory response in human keratinocytes by regulating the MAPKs/AP-1, NFκB, and NFAT signaling pathways. Molecules. 2021;26(4):869,1-14. DOI: 10.3390/molecules26040869.

Jeong J, Bae K, Kim S-G, Kwak D, Moon Y-J, Choi C-H, et al. Anti-osteoarthritic effects of chondroT in a rat model of collagenase-induced osteoarthritis. BMC Complement Altern Med. 2018;18(1):131-141. DOI: 10.1186/s12906-018-2149-1.

Loeser RF, Goldring SR, Scanzello CR, Goldring MB. Osteoarthritis: a disease of the joint as an organ. Arthritis Rheum. 2012;64(6):1697-1707. DOI: 10.1002/art.34453.

Abramson SB, Attur M. Developments in the scientific understanding of osteoarthritis. Arthritis Res Ther. 2009;11(3):227-236. DOI: 10.1186/ar2655.

Abdel-Rahman RF, Abd-Elsalam RM, Amer MS, El-Desoky AM, Mohamed SO. Manjarix attenuated pain and joint swelling in a rat model of monosodium iodoacetate-induced osteoarthritis. Food Funct. 2020;11(9):7960-7972. DOI: 10.1039/d0fo01297a.

Lee YM, Son E, Kim S-H, Kim OS, Kim D-S. Anti-inflammatory and anti-osteoarthritis effect of Mollugo pentaphylla extract. Pharm Biol. 2019;57(1):74-81. DOI: 10.1080/13880209.2018.1557700.

Refaat AT, Shahat AA, Ehsan NA, Yassin N, Hammouda F, Tabl EA, et al. Phytochemical and biological activities of Crataegus sinaica growing in Egypt. Asian Pac J Trop Med. 2010;3(4):257-261. DOI: 10.1016/S1995-7645(10)60062-4.

Barros L, Carvalho AM, Ferreira ICFR. Comparing the composition and bioactivity of Crataegus monogyna flowers and fruits used in folk medicine. Phytochem Anal. 2011;22(2):181-188. DOI: 10.1002/pca.1267.

Abdel-Rahman R, Eldesoky A, Handoussa H, Meselhy MR, Asaad G, El-Mekkawy S. LC-MS-based chemical profiling and in-vivo evaluation of the anti-inflammatory and anti-nociceptive activities of the defatted methanolic extract of Crataegus sinaica (Rosaceae) fruits. Egypt J Chem. 2022;65(4):161-173. DOI: 10.21608/ejchem.2021.94061.4429.

Yassin NZ, El-Shenawy SM, Abdel-Rahman RF, Yakoot M, Hassan M, Helmy S. Effect of a topical copper indomethacin gel on inflammatory parameters in a rat model of osteoarthritis. Drug Des Devel Ther. 2015;9:1491-1498. DOI: 10.2147/DDDT.S79957.

Hattori K, Ikeuchi K, Morita Y, Takakura Y. Quantitative ultrasonic assessment for detecting microscopic cartilage damage in osteoarthritis. Arthritis Res Ther. 2005;7(1):R38-R46. DOI: 10.1186/ar1463.

Kellgren JH, Lawrence JS. Radiological assessment of osteo-arthrosis. Ann Rheum Dis. 1957;16(4):494-502. DOI: 10.1136/ard.16.4.494.

Gerwin N, Bendele AM, Glasson S, Carlson CS. The OARSI histopathology initiative - recommendations for histological assessments of osteoarthritis in the rat. Osteoarthritis Cartilage. 2010;18 Suppl 3: S24-S34. DOI: 10.1016/j.joca.2010.05.030.

Pritzker KPH, Gay S, Jimenez SA, Ostergaard K, Pelletier J-P, Revell PA, et al. Osteoarthritis cartilage histopathology: grading and staging. Osteoarthritis Cartilage. 2006;14(1):13-29. DOI: 10.1016/j.joca.2005.07.014.

Ogaly HA, Alsherbiny MA, El Badawy SA, Abd-Elsalam RM, Guang Li C, Azouz AA. Gastroprotective effects and metabolomic profiling of chasteberry fruits against indomethacin-induced gastric injury in rats. J Funct Foods. 2021;86(1):104732-104747. DOI: 10.1016/j.jff.2021.104732.

Ogaly HA, Abdel-Rahman RF, Elbaset Mohamed MA, Ahmed-Farid OA, Khattab MS, Abd-Elsalam RM. Thymol ameliorated neurotoxicity and cognitive deterioration in a thioacetamide-induced hepatic encephalopathy rat model; involvement of the BDNF/CREB signaling pathway. Food Funct. 2022;13(11): 6180-6194.

Abdel-Hameed E-SS, Bazaid SA, Salman MS. Characterization of the phytochemical constituents of Taif rose and its antioxidant and anticancer activities. Biomed Res Int. 2013;2013:345465. DOI:10.1155/2013/345465.

Rocchetti G, Senizza B, Zengin G, Mahomodally MF, Senkardes I, Lobine D, et al. Untargeted metabolomic profiling of three Crataegus species (hawthorn) and their in vitro biological activities. J Sci Food Agric. 2020;100(5):1998-2006. DOI: 10.1002/jsfa.10216.

Elsadig Karar MG, Kuhnert N. UPLC-ESI-Q-TOF-MS/MS Characterization of phenolics from Crataegus monogyna and Crataegus laevigata (Hawthorn) leaves, fruits and their herbal derived drops (Crataegutt tropfen). J Chem Biol Ther. 2016;1(1):102-124. DOI: 10.4172/2572-0406.1000102.

Li Z-H, Guo H, Xu W-B, Ge J, Li X, Alimu M, et al. Rapid Identification of flavonoid constituents directly from PTP1B inhibitive extract of raspberry (Rubus idaeus L.) leaves by HPLC-ESI-QTOF-MS-MS. J Chromatogr Sci. 2016;54(5):805-810. DOI: 10.1093/chromsci/bmw016.

Ao N, Qu Y, Deng Y, Cai Q, Suo T, Zheng Y. Chemical basis of hawthorn processed with honey protecting against myocardial ischaemia. Food Funct. 2020;11(4):3134-3143. DOI: 10.1039/c9fO02406a.

Takahashi I, Matsuzaki T, Kuroki H, Hoso M. Induction of osteoarthritis by injecting monosodium iodoacetate into the patellofemoral joint of an experimental rat model. PLoS One. 2018;13(4)):e0196625,1-19. DOI: 10.1371/journal.pone.0196625.

Shin IS, Lee MY, Lim HS, Ha H, Seo CS, Kim J-C, et al. An extract of Crataegus pinnatifida fruit attenuates airway inflammation by modulation of matrix metalloproteinase-9 in ovalbumin induced asthma. PLoS One. 2012;7(9):e45734-1-16. DOI: 10.1371/journal.pone.0045734.

Tadić VM, Dobrić S, Marković GM, Dordević SM, Arsić IA, Menković NR, et al. Anti-inflammatory, gastroprotective, free-radical-scavenging, and antimicrobial activities of hawthorn berries ethanol extract. J Agric Food Chem. 2008;56(17):7700-7709. DOI: 10.1021/jf801668c.

Ashraf S, Mapp PI, Shahtaheri SM, Walsh DA. Effects of carrageenan induced synovitis on joint damage and pain in a rat model of knee osteoarthritis. Osteoarthritis Cartilage. 2018;26(10):1369-1378. DOI: 10.1016/j.joca.2018.07.001.

Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis. Toxicol Pathol. 2003;31:619-624. DOI: 10.1080/01926230390241800.

Bor Z, Arslan R, Bektaş N, Pirildar S. Donmez AA. Antinociceptive, antiinflammatory, and antioxidant activities of the ethanol extract of Crataegus orientalis leaves. Turk J Med Sci. 2012;42(2):315–324. DOI: 10.3906/sag-1011-1304.

Alsayari A, Ghazwani M, Almaghaslah D, Alhamhoom Y, Louis J, Altohami J, et al. Potential analgesic and diuretic activity of Crataegus oxyacantha Linn. Res J Pharm Technol. 2018;11(6):2476-2482. DOI: 10.5958/0974-360X.2018.00457.2.

Li G, Yin J, Gao J, Cheng TS, Pavlos NJ, Zhang C, et al. Subchondral bone in osteoarthritis: insight into risk factors and microstructural changes. Arthritis Res Ther. 2013;15(6):223-234. DOI: 10.1186/ar4405.

Goldring MB. Osteoarthritis and cartilage: the role of cytokines. Curr Rheumatol Rep. 2000;2(6):459-465. DOI: 10.1007/s11926-000-0021-y.

Kim S-J, Um J-Y, Lee J-Y. Anti-inflammatory activity of hyperoside through the suppression of nuclear factor-κB activation in mouse peritoneal macrophages. Am J Chin Med. 2011;39(1):171-181. DOI: 10.1142/S0192415X11008737.

Shen J, Li S, Chen D. TGF-β signaling and the development of osteoarthritis. Bone Res. 2014;2:14002,1-18. DOI: 10.1038/boneres.2014.2.

Hamza AA, Lashin FM, Gamel M, Hassanin SO, Abdalla Y, Amin A. Hawthorn herbal preparation from Crataegus oxyacantha attenuates in vivo carbon tetrachloride -induced hepatic fibrosis via modulating oxidative stress and inflammation. Antioxidants (Basel, Switzerland). 2020;9(12):1173,1-17. DOI: 10.3390/antiox9121173.

Liu G, Cui Z, Gao X, Liu H, Wang L, Gong J, et al. Corosolic acid ameliorates non-alcoholic steatohepatitis induced by high-fat diet and carbon tetrachloride by regulating TGF-β1/Smad2, NF-κB, and AMPK signaling pathways. Phytother Res. 2021;35(9):5214-5226. DOI: 10.1002/ptr.7195.

Ravindran ASK, Venkatabalasubramanian S, Manickam R, Anusuyadevi M, Swaminathan JK. Plausible computational insights and new atomic-level perspective of epicathechin gallate from Crataegus oxycantha extract in preventing caspase 3 activation in conditions like post-myocardial infarction. J Biomol Struct Dyn. 2020;0:1-16 DOI: 10.1080/07391102.2020.1847196.

Liu S, Sui Q, Zou J, Zhao Y, Chang X. Protective effects of hawthorn (Crataegus pinnatifida) polyphenol extract against UVB-induced skin damage by modulating the p53 mitochondrial pathway in vitro and in vivo. J Food Biochem. 2019;43(2):e12708-1-12. DOI: 10.1111/jfbc.12708.


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