Hydroethanolic extract of Lavandula angustifolia ameliorates vincristine-induced peripheral neuropathy in rats

Shamim Sahranavard , Mona Khoramjouy, Mahsima Khakpash, Seyyed Ahmad Askari, Mehrdad Faizi , Mahmoud Mosaddegh

Abstract


Background and purpose: Peripheral neuropathy is one of the most common adverse effects of cancer chemotherapy. Vincristine is prescribed to treat a variety of carcinomas, including lymphoma and leukemia, and may cause progressive peripheral neuropathy due to the damage of microtubules and mitochondria of neurons and affects inflammatory processes. This study was designed to evaluate the effects of Lavandula angustifolia hydroalcoholic extract (LHE) of aerial part on vincristine-induced peripheral neuropathy in a rat model.

Experimental approach: Neuropathy was induced in rats by daily intraperitoneal administration of vincristine (0.1 mg/kg for 2 weeks). Following the induction of neuropathy, animals were treated with the LHE (100, 200, and 400 mg/kg, p.o.) or pregabalin (20 mg/kg, IP) for 2 weeks, and their responses to vincristine-induced hyperalgesia and locomotor impairment were measured.

Findings/Results: LHE, at the dose of 400 mg/kg, showed analgesic effects in response to thermal hyperalgesia, tactile allodynia, and gait impairment. Also, pregabalin (20 mg/kg, IP) improved the symptoms of vincristine-induced peripheral neuropathy.

Conclusions and implications: According to the results, we can conclude that LHE alleviates neuropathic symptoms of vincristine and the effect is probably related to the presence of phenols and flavonoids in the extract.

 

 


Keywords


Lavandula angustifolia; Neuropathy; Vincristine.

Full Text:

PDF

References


Jaggi AS, Singh N. Mechanisms in cancer-chemotherapeutic drugs-induced peripheral neuropathy. Toxicology. 2012; 291(1-3):1-9.

DOI: 10.1016/j.tox.2011.10.019.

Seretny M, Currie GL, Sena ES, Ramnarine S, Grant R, MacLeod MR, et al. Incidence, prevalence, and predictors of chemotherapy-induced peripheral neuropathy: a systematic review and meta-analysis. Pain. 2014;155(12):2461-2470.

DOI: 10.1016/j.pain.2014.09.020.

Tanner KD, Levine JD, Topp KS. Microtubule disorientation and axonal swelling in unmyelinated sensory axons during vincristine-induced painful neuropathy in rat. J Comp Neurol. 1998;395(4): 481-492.

PMID: 9619501.

Chiba T, Oka Y, Sashida H, Kanbe T, Abe K, Utsunomiya I, et al. Vincristine-induced peripheral neuropathic pain and expression of transient receptor potential vanilloid 1 in rat. J Pharmacol Sci. 2017;133(4):254-260.

DOI: 10.1016/j.jphs.2017.03.004.

Argyriou AA, Kyritsis AP, Makatsoris T, Kalofonos HP. Chemotherapy-induced peripheral neuropathy in adults: a comprehensive update of the literature. Cancer Manag Res. 2014;6:135-147.

DOI: 10.2147/CMAR.S44261.

Kaur G, Jaggi AS, Singh N. Exploring the potential effect of Ocimum sanctum in vincristine-induced neuropathic pain in rats. J Brachial Plex Peripher Nerve Inj. 2010;5:3-11.

DOI: 10.1186/1749-7221-5-3.

Babu A, Prasanth KG, Balaji B. Effect of curcumin in mice model of vincristine-induced neuropathy. Pharm Biol. 2015;53(6):838-848.

DOI: 10.3109/13880209.2014.943247.

Linglu D, Yuxiang L, Yaqiong X, Ru Z, Lin M, Shaoju J, et al. Antinociceptive effect of matrine on vincristine-induced neuropathic pain model in mice. Neurol Sci. 2014;35(6):815-821.

DOI: 10.1007/s10072-013-1603-6.

El-Gabbas Z, Bezza K, Laadraoui J, Ait Laaradia M, Kebbou A, Oufquir S, et al. Salvia officinalis, rosmarinic and caffeic acids attenuate neuropathic pain and improve function recovery after sciatic nerve chronic constriction in mice. Evid Based Complement Alternat Med. 2019;2019:1702378.

DOI: 10.1155/2019/1702378.

Nurmikko TJ, Serpell MG, Hoggart B, Toomey PJ, Morlion BJ, Haines D. Sativex successfully treats neuropathic pain characterised by allodynia: a randomised, double-blind, placebo-controlled clinical trial. Pain. 2007;133(1-3):210-220.

DOI: 10.1016/j.pain.2007.08.028.

Liu Y, Zhu G, Han L, Liu J, Ma T, Yu H. Clinical study on the prevention of oxaliplatin-induced neurotoxicity with guilongtongluofang: results of a randomized, double-blind, placebo-controlled tria. Evid Based Complement Alternat Med. 2013;2013:541217.

DOI: 10.1155/2013/541217.

Akhondzadeh S, Kashani L, Fotouhi A, Jarvandi S, Mobaseri M, Moin M, et al. Comparison of Lavandula angustifolia Mill. tincture and imipramine in the treatment of mild to moderate depression: a double-blind, randomized trial. Prog Neuropsychopharmacol Biol Psychiatry. 2003;27(1):123-127.

DOI: 10.1016/s0278-5846(02)00342-1.

Kashani MS, Tavirani MR, Talaei SA, Salami M. Aqueous extract of lavender (Lavandula angustifolia) improves the spatial performance of a rat model of Alzheimer’s disease. Neurosci Bull. 2011; 27(2):99-106.

DOI: 10.1007/s12264-011-1149-7.

Hajhashemi V, Ghannadi A, Sharif B. Antiinflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia Mill. J Ethnopharmacol. 2003;89(1):67-71.

DOI: 10.1016/s0378-8741(03)00234-4.

Prusinowska R, Śmigielski KB. Composition, biological properties and therapeutic effects of lavender (Lavandula angustifolia L.). A review. Herba Polonica. 2014;60(2):56-66.

DOI: 10.2478/hepo-2014-0010.

Naghibi F, Mosaddegh M, Mohammadi Motamed S, Ghorbani A. Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. Iran J Pharm Res. 2005;4(2):63-79.

DOI: 10.22037/IJPR.2010.619.

Aghili-Alavi-Khorasani MH, Shams-Ardakani MR, Rahimi R, Farjadmand F. Makhzan-al-Adwieh. Sabz-arang and Tehran University of Medical Sciences, Tehran. 2009. pp: 93-94.

Siau C, Bennett GJ. Dysregulation of neuronal calcium homeostasis in chemotherapy-evoked painful peripheral neuropathy. Anesth Analg. 2006;102(5):1485-1490.

DOI: 10.1213/01.ane.0000204318.35194.ed.

Khalilzadeh M, Panahi G, Rashidian A, Hadian MH, Abdollahi A, Afshari K, et al. The protective effects of sumatriptan on vincristine-induced peripheral neuropathy in a rat model. Neurotoxicology. 2018;67:279-286.

DOI: 10.1016/j.neuro.2018.06.012.

Abdollahnejad F, Mosaddegh M, Kamalinejad M, Mirnajafi-Zadeh J, Najafi F, Faizi M. Investigation of sedative and hypnotic effects of Amygdalus communis L. extract: behavioral assessments and EEG studies on rat. J Nat Med. 2016;70(2):190-197.

DOI: 10.1007/s11418-015-0958-9.

Zimcikova E, Simko J, Karesova I, Kremlacek J, Malakova J. Behavioral effects of antiepileptic drugs in rats: are the effects on mood and behavior detectable in open-field test? Seizure. 2017;52:35-40.

DOI: 10.1016/j.seizure.2017.09.015.

Chu Q, Song A, Zhao R, Liu J, Shi H, Liu P, et al. Establishment and evaluation of a compound fear behavior model of Tourette's syndrome in rats. Ann Transl Med. 2021;9(18):1469-1477.

DOI: 10.21037/atm-21-4515.

Eddy NB, Touchberry CF, Lieberman JE. Synthetic analgesics: I. Methadone isomers and derivatives. J Pharmacol Exp Ther. 1950;98(2):121-137.

PMID: 15422505.

Gabriel AF, Marcus MAE, Walenkamp GHIM, Joosten EAJ. The CatWalk method: assessment of mechanical allodynia in experimental chronic pain. Behav Brain Res. 2009;198(2):477-480.

DOI: 10.1016/j.bbr.2008.12.018.

Chen W, Xia M, Guo C, Jia Z, Wang J, Li C, et al. Modified behavioural tests to detect white matter injury- induced motor deficits after intracerebral haemorrhage in mice. Sci Rep. 2019;9:16958,1-11.

DOI: 10.1038/s41598-019-53263-6.

Ziaei A, Sahranavard SH, Gharagozlou MJ, Faizi M. Preliminary investigation of the effects of topical mixture of Lawsonia inermis L. and Ricinus communis L. leaves extract in treatment of osteoarthritis using MIA model in rats. Daru. 2016;24(1):12-21.

DOI: 10.1186/s40199-016-0152-y.

Geis C, Beyreuther BK, Stohr T, Sommer C. Lacosamide has protective disease modifying properties in experimental vincristine neuropathy. Neuropharmacology. 2011;61(4):600-607.

DOI: 10.1016/j.neuropharm.2011.05.001.

Park HJ, Lee HG, Kim YS, Lee JY, Jeon J, Park C, et al. Ginkgo biloba extract attenuates hyperalgesia in a rat model of vincristine-induced peripheral neuropathy. Anesth Analg. 2012;115(5):1228-1233.

DOI: 10.1213/ANE.0b013e318262e170.

Wahdan SA, Elsherbiny DA, Azab SS, El-Demerdash E. Piceatannol ameliorates behavioural, biochemical and histological aspects in cisplatin-induced peripheral neuropathy in rats. Basic Clin Pharmacol Toxicol. 2021;129(6):486-495.

DOI: 10.1111/bcpt.13643.

Li AL, Crystal JD, Lai YY, Sajdyk TJ, Renbarger JL, Hohmann AG. An adolescent rat model of vincristine-induced peripheral neuropathy. Neurobiol Pain. 2021;10:100077,1-9.

DOI: 10.1016/j.ynpai.2021.100077.

Mangaiarkkarasi A, Rameshkannan S, Meher-Ali R. Effect of gabapentin and pregabalin in rat model of taxol induced neuropathic pain. J Clin Diagn Res. 2015;9(5):FF11-14.

DOI: 10.7860/JCDR/2015/13373.5955.

Sanna MD, Les F, Lopez V, Galeotti N. Lavender (Lavandula angustifolia Mill.) essential oil alleviates neuropathic pain in mice with spared nerve injury. Front Pharmacol. 2019;10:472-484.

DOI: 10.3389/fphar.2019.00472.

Donatello NN, Emer AA, Salm DC, Ludtke DD, Bordignon SASR, Ferreira JK, et al. Lavandula angustifolia essential oil inhalation reduces mechanical hyperalgesia in a model of inflammatory and neuropathic pain: The involvement of opioid and cannabinoid receptors. J Neuroimmunol. 2020;340:577145.

DOI: 10.1016/j.jneuroim.2020.577145.

Nurzyńska-Wierdak R, Zawiślak G. Chemical composition and antioxidant activity of lavender (Lavandula angustifolia Mill.) aboveground parts. Acta Sci Pol Hortorum Cultus. 2016; 15(5):225-241.

Uddin MS, Mamun AA, Rahman MA, Kabir MT, Alkahtani S, Alanazi IS, et al. Exploring the promise of flavonoids to combat neuropathic pain: from molecular mechanisms to therapeutic implications. Front Neurosci. 2020;14:478-495.

DOI: 10.3389/fnins.2020.00478.

Azevedo MI, Pereira AF, Nogueira RB, Rolim FE, Brito GAC, Wong DVT, et al. The antioxidant effects of the flavonoids rutin and quercetin inhibit oxaliplatin-induced chronic painful peripheral neuropathy. Mol Pain. 2013;9:53-66.

DOI: 10.1186/1744-8069-9-53.

Cherng CH, Lee KC, Chien CC, Chou KY, Cheng YC, Hsin ST, et al. Baicalin ameliorates neuropathic pain by suppressing HDAC1 expression in the spinal cord of spinal nerve ligation rats. J Formos Med Assoc. 2014;113(8):513-520.

DOI: 10.1016/j.jfma.2013.04.007.

Hagenacker T, Hillebrand I, Wissmann A, Büsselberg D, Schäfers M. Anti-allodynic effect of the flavonoid myricetin in a rat model of neuropathic pain: involvement of p38 and protein kinase C mediated modulation of Ca2+ channels. Eur J Pain. 2010;14(10):992-998.

DOI: 10.1016/j.ejpain.2010.04.005.

Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci. 2004;96(3):229-245.

DOI: 10.1254/jphs.crj04003x.


Refbacks

  • There are currently no refbacks.


Creative Commons LicenseThis work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.