Development, physicochemical characterization, and antimicrobial evaluation of niosomal myrtle essential oil

Mahboobeh Raeiszadeh, Abbas Pardakhty, Fariba Sharififar, Alireza Farsinejad, Mehrnaz Mehrabani, Hossein Hosseini-nave, Mitra Mehrabani

Abstract


Myrtus communis (myrtle) is well known for its therapeutic effects pertaining to the major secondary metabolites including essential oils (EOs). EOs are composed of volatile compounds and simply evaporate or decompose leading to their instability. Preparation of EOs niosomal formulation may be a promising approach to deal with these obstacles. Niosomal formulations of myrtle essential oil (nMEO) were provided using non-ionic surfactants and cholesterol (Chol). In the next steps, vesicle size, zeta potential, percentage of entrapment efficiency (EE%) and physical stability of nMEO were investigated. Finally, the effect of myrtle essential oil (MEO) and nMEO on microbial growth inhibition were assessed. Values for nMEO size and zeta potential ranged from 6.17 ± 0.32 to 7.24 ± 0.61 (µm) and -20.41 ± 0.17 to -31.75 ± 0.45 (mV), respectively. Higher degrees of EE% were obtained by F6 formulation (Span/Tween 60:Chol (50:50 molar ratio)). Moreover, niosomes have been reported to be stable at 4 °C during a three-month time period. It was revealed that nMEO F6 formulation inhibited growth of Staphylococcus aureus, Staphylococcus epidermidis, Serratia marcescens, and Bacillus subtilis at concentrations lower than that of MEO. Overall, it was found that stable multilamellar vesicles were formed in the presence of 0.5% MEO and F6 formulation. This formulation also exhibited better antibacterial activity than MEO.


Keywords


Antibacterial activity; Essential oil; Myrtle, Myrtus communis; Niosome

Full Text:

PDF

References


Aleksic V, Knezevic P. Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L. Microbiol Res. 2014;169(4):240-254.

Alipour G, Dashti S, Hosseinzadeh H. Review of pharmacological effects of Myrtus communis L. and its active constituents. Phytother Res. 2014;28(8):1125-1136.

Avicenna. Canon of Medicine. Tehran, Iran: Soroosh Publisher; 1986. pp. 56-58.

Gardeli A, Papageorgiou V, Mallouchos A, Kibouris T, Komaitis M. Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: evaluation of antioxidant capacity of methanolic extracts. Food Chem. 2008;107(3):1120-1130.

Edris AE. Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytother Res. 2007;21(4):308-323.

Hosseini SF, Zandi M, Rezaei M, Farahmandghavi F. Two-step method for encapsulation of oregano essential oil in chitosan nanoparticles: preparation, characterization and in vitro release study. Carbohydr Polym. 2013;95(1):50-56.

Manosroi A, Kietthanakorn BO, Chankhampan C, Khositsuntiwong N, Manosroi W, Abe M, et al. Physical characteristics and biological activities of Thai flower extracts loaded in niosomes. Month. 2013;40(4):603-617.

Zarkesh Kh, Khazaeli P, Pardakhty A, Rezaifar M. Preparation and physicochemical characterization of topical niosomal formulation of minoxidil and tretinoin. Glob J Pharmaceu Sci. 2017;3(2):001-006.

Nematollahi MH, Torkzadeh-Mahanai M, Pardakhty A, Ebrahimi Meimand HA, Asadikaram G. Ternary complex of plasmid DNA with NLS-Mu-Mu protein and cationic niosome for biocompatible and efficient gene delivery: a comparative study with protamine and lipofectamine. Artif Cells Nanomed Biotechnol. 2017:1-11.

Puras G, Mashal M, Zárate J, Agirre M, Ojeda E, Grijalvo S, et al. A novel cationic niosome formulation for gene delivery to the retina. J Control Release. 2014;174:27-36.

Basiri L, Rajabzadeh G, Bostan A. α-Tocopherol-loaded niosome prepared by heating method and its release behavior. Food Chem. 2017;221:620-628.

Budhiraja A, Dhingra G. Development and characterization of a novel antiacne niosomal gel of rosmarinic acid. Drug Deliv. 2015;22(6):723-730.

Patel J, Ketkar S, Patil S, Fearnley J, Mahadik KR, Paradkar AR. Potentiating antimicrobial efficacy of propolis through niosomal-based system for administration. Integr Med Res. 2015;4(2):94-101.

Junyaprasert VB, Singhsa P, Suksiriworapong J, Chantasart D. Physicochemical properties and skin permeation of Span 60/Tween 60 niosomes of ellagic acid. Int J Pharm. 2012;423(2):303-311.

Manosroi A, Chutoprapat R, Abe M, Manosroi W, Manosroi J. Anti-aging efficacy of topical formulations containing niosomes entrapped with rice bran bioactive compounds. Pharm Biol. 2012;50(2):208-224.

Weckesser S, Engel K, Simon-Haarhaus B, Wittmer A, Pelz K, Schempp CM. Screening of plant extracts for antimicrobial activity against bacteria and yeasts with dermatological relevance. Phytomedicine. 2007;14(7-8):508-516.

Basualdo C, Sgroy V, Finola MS, Marioli JM. Comparison of the antibacterial activity of honey from different provenance against bacteria usually isolated from skin wounds. Vet Microbiol. 2007;124(3-4):375-381.

Ghasemi dehkordi N, Sajadi SE, Ghanadi A, Amanzadeh Y, Azadbakht M, Asghari Gh, et al. Iranian Herbal Pharmacopeia. 1st ed. Tehran: Iranian Ministry of Health and Medical Education Publications; 2002. pp. 23-24.

Adams RP. Identification of Essential Oil Components by Gas Chromatography/

Quadrupole Mass Spectroscopy. 3rd ed. Carol Stream, Illinois: Allured Publishing Corporation; 2004. pp. 261-263 .

Nematollahi MH, Pardakhty A, Torkzadeh-Mahanai M, Mehrabani M, Asadikaram Gh. Changes in physical and chemical properties of niosome membrane induced by cholesterol: a promising approach for niosome bilayer intervention. RSC Adv. 2017;7(78):49463-49472.

Khazaeli P, Pardakhty A, Shoorabi H. Caffeine-loaded niosomes: characterization and in vitro release studies. Drug Deliv. 2007;14(7):447-452.

Kamble B, Talreja S, Gupta A, Patil D, Pathak D, Moothedath I, et al. Development and biological evaluation of Gymnema sylvestre extract-loaded nonionic surfactant-based niosomes. Nanomedicine (Lond). 2013;8(8):1295-1305.

Valgas C, de Souza SM, Smânia EFA, Smânia Jr A. Screening methods to determine antibacterial activity of natural products. Braz J Microbiol. 2007;38(2):369-380.

Prabuseenivasan S, Jayakumar M, Ignacimuthu S. In vitro antibacterial activity of some plant essential oils. BMC complement Altern Med. 2006;6(1):39.

Lee SC, Lee KE, Kim JJ, Lim SH. The effect of cholesterol in the liposome bilayer on the stabilization of incorporated retinol. J Liposome Res. 2005;15(3-4):157-166.

Varshosaz J, Taymouri S, Pardakhty A, Asadi-Shekaari M, Babaee A. Niosomes of ascorbic acid and α-tocopherol in the cerebral ischemia-reperfusion model in male rats. BioMed Res Int. 2014;2014:816103. doi:10.1155/2014/816103.

Moazeni E, Gilani K, Sotoudegan F, Pardakhty A, Najafabadi AR, Ghalandari R, et al. Formulation and in vitro evaluation of ciprofloxacin containing niosomes for pulmonary delivery. J Microencapsul. 2010;27(7):618-627.

Randrianarivelo R, Sarter S, Odoux E, Brat P, Lebrun M, Romestand B, et al. Composition and antimicrobial activity of essential oils of Cinnamosma fragrans. Food Chem. 2009;114(2):680-684.

Carson CF, Riley TV. Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. J Appl Bacteriol. 1995;78(3):264-269.

Mahmoudvand H, Ezzatkhah F, Sharififar F, Sharifi I, Dezaki ES. Antileishmanial and cytotoxic effects of essential oil and methanolic extract of Myrtus communis L. Korean J Parasitol. 2015;53(1):21-27.

Mahboubi M, Bidgoli FG. In vitro synergistic efficacy of combination of amphotericin B with Myrtus communis essential oil against clinical isolates of Candida albicans. Phytomedicine. 2010;17(10):771-774.

Senatore F, Formisano C, Napolitano F, Rigano D, Özcan M. Chemical composition and antibacterial activity of essential oil of Myrtus communis L. growing wild in Italy and Turkey. J Essent Oil Bear Plants. 2006;9(2):162-169.

Lai F, Wissing SA, Müller RH, Fadda AM. Artemisia arborescens L essential oil-loaded solid lipid nanoparticles for potential agricultural application: preparation and characterization. AAPS PharmSciTech. 2006;7(1):E10.

Liolios CC, Gortzi O, Lalas S, Tsaknis J, Chinou I. Liposomal incorporation of carvacrol and thymol isolated from the essential oil of Origanum dictamnus L. and in vitro antimicrobial activity. Food Chem. 2009;112(1):77-83.

Varshosaz J, Pardakhty A, Hajhashemi VI, Najafabadi AR. Development and physical characterization of sorbitan monoester niosomes for insulin oral delivery. Drug Deliv. 2003;10(4):251-262.

Hao YM, Li K. Entrapment and release difference resulting from hydrogen bonding interactions in niosome. Int J Pharm. 2011;403(1-2):245-253.

Ruckmani K, Sankar V. Formulation and optimization of zidovudine niosomes. AAPS PharmSciTech. 2010;11(3):1119-1127.

Guinedi AS, Mortada ND, Mansour S, Hathout RM. Preparation and evaluation of reverse-phase evaporation and multilamellar niosomes as ophthalmic carriers of acetazolamide. Int J Pharm. 2005;306(1-2):71-82.

Bayindir ZS, Yuksel N. Characterization of niosomes prepared with various nonionic surfactants for paclitaxel oral delivery. J Pharm Sci. 2010;99(4):2049-2060.

Lawrence J, Chauhan S, Lawrence SM, Barlow D. The formation, characterization and stability of non-ionic surfactant vesicles. STP Pharm Sci. 1996;6(1):49-60.

Salvagnini LE, Oliveira JRS, Santos LEd, Moreira RRD, Pietro RCLR. Evaluation of the antibacterial activity of Myrtus communis L.(Myrtaceae) leaves. Rev Bras Farmacogn. 2008;18(2):241-244.

Yadegarinia D, Gachkar L, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I. Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry. 2006;67(12):1249-1255.

Kopermsub P, Mayen V, Warin C. Nanoencapsulation of nisin and ethylene diamine tetra acetic acid in niosomes and their antibacterial activity. J Sci Res. 2012;4(2):457-465.

Akbari V, Abedi D, Pardakhty A, Sadeghi-Aliabadi H. Ciprofloxacin nano-niosomes for targeting intracellular infections: an in vitro evaluation. J Nanopart Res. 2013;15(4):1556-1570.


Refbacks

  • There are currently no refbacks.


Creative Commons LicenseThis work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.