Effects of Cornus mas fruit hydro-methanolic extract on serum antioxidants, lipid profile, and hematologic parameters following cisplatin-induced changes in rats

Atefe Mohammadzadeh Vardin, Bita Abdollahi, Morteza Kosari-Nasab, Mehran Mesgari Abbasi

Abstract


Cisplatin (Cis) has serious adverse side-effects that limit its clinical use. The mechanism underlying the effects is complex, including mitochondrial oxidative stress and inflammation. This study investigated whether Cornus mas, a fruit with high antioxidant contents, hydro-methanolic extract (CME) can modulate the cisplatin-induced changes. Forty Wistar rats were divided into a control group, Cis group, CME group, CME 300 + Cis group, and the CME 700 + Cis group. After the intervention, blood samples were taken for biochemical and hematological analysis. CME analysis showed noticeable total phenol and total antioxidant contents. The plasma glutathione peroxidase and catalase levels were significantly decreased and malondialdehyde and blood hemoglobin levels were significantly increased in the Cis group, which were reversed to the control levels in the CME + Cis groups. In the CME group, the red blood cell count was significantly lower and the red cell distribution width and hemoglobin distribution width levels were significantly higher. In the Cis-treated group, white blood cells, neutrophils, monocytes, basophils, and large unstained cells were significantly increased and lymphocytes were significantly decreased when compared with the control group that was reached to non-significant levels in CME 700 + Cis group. The blood cholesterol and high density lipoprotein in all CME-treated groups were significantly decreased. The eosinophils increased in the CME group significantly. The results showed considerable total antioxidant and total phenol contents and relative protective effects of CME against Cis-induced antioxidant and hematologic changes in rats.


Keywords


Antioxidant; Cisplatin; Cornus mas; Lipid profile; Hematologic parameters

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Pabla N, Dong Z. Cisplatin nephrotoxicity: mechanisms and renoprotective strategies. Kidney Int. 2008;73(9):994-1007.

Miller RP, Tadagavadi RK, Ramesh G, Reeves WB. Mechanisms of cisplatin nephrotoxicity. Toxins (Basel). 2010;2(11):2490-518.

Almaghrabi OA. Molecular and biochemical investigations on the effect of quercetin on oxidative stress induced by cisplatin in rat kidney. Saudi J Biol Sci. 2015;22(2):227-231.

Dasari S, Tchounwou PB. Cisplatin in cancer therapy: molecular mechanisms of action. Eur J Pharmacol. 2014;740:364-378.

Ozkok A, Edelstein CL. Pathophysiology of cisplatin-induced acute kidney injury. Biomed Res Int. 2014;2014:967826.

Pabla N, Dong Z. Curtailing side effects in chemotherapy: a tale of PKCdelta in cisplatin treatment. Oncotarget. 2012;3(1):107-111.

Zarei L, Shahrooz R, Sadrkhanlou R, Malekinejad H, Ahmadi A, Bakhtiary Z. Protective effects of cornus mas extract on in vitro fertilization potential in methotrexate treated male mice. Vet Res Forum. 2015;6(1):55-61.

Abdollahi B, Mesgari Abbasi M, Zakeri Milani P, Nourdadgar AS, Banan Khojasteh SM, Nejati V. Hydro-methanolic extract of cornus MAS L. and blood glucose, lipid profile and hematological parameters of male rats. Iran Red Crescent Med J. 2014;16(5):e17784.

Alavian SM, Banihabib N, Es Haghi M, Panahi F. Protective effect of Cornus mas fruits extract on serum biomarkers in CCl4-induced hepatotoxicity in male rats. Hepat Mon. 2014;14(4):e10330.

Vareed SK, Reddy MK, Schutzki RE, Nair MG. Anthocyanins in Cornus alternifolia, Cornus controversa, Cornus kousa and Cornus florida fruits with health benefits. Life Sci. 2006;78(7):777-784.

Francik R, Kryczyk J, Krosniak M, Berkoz M, Sanocka I, Francik S. The neuroprotective effect of cornus MAS on brain tissue of Wistar rats. Scientific World J. 2014;2014: Article ID 847368, 9 pages.

Ghasemi Pirbalouti A, Siahpoosh A, Setayesh M, Craker L. Antioxidant activity, total phenolic and flavonoid contents of some medicinal and aromatic plants used as herbal teas and condiments in Iran. J Med Food. 2014;17(10):1151-1157.

Mesgari Abbasi M, Heidari R, Amini afshar R, Zakeri Milani P, Ghamarzad Shishavan N. Effects of pomegranate seed methanolic extract on methotrexateinduced changes in rat liver antioxidant compounds. Curr Top Nutraceutical Res. 2015;13(3):153-159.

Vador N, Vador B, Hole R. Simple spectrophotometric methods for standardizing ayurvedic formulation. Indian J Pharm Sci. 2012;74(2):161-163.

Eshaghi M, Zare S, Banihabib N, Nejati V, Farokhi F, Mikaili P. Cardioprotective effect of cornus mas fruit extract against carbon tetrachloride induced-cardiotoxicity in albino rats. J Basic Appl Sci Res. 2012;2(11):11106-11114.

Naghizadeh B, Boroushaki MT, Vahdati Mashhadian N, Mansouri MT. Protective effects of crocin against cisplatin-induced acute renal failure and oxidative stress in rats. Iran Biomed J. 2008;12(2):93-100.

Tikoo K, Bhatt DK, Gaikwad AB, Sharma V, Kabra DG. Differential effects of tannic acid on cisplatin induced nephrotoxicity in rats. FEBS Lett. 2007;581(10):2027-2035.

Ellis PA, Fitzharris BM, George PM, Robinson BA, Atkinson CH, Colls BM. Fasting plasma lipid measurements following cisplatin chemotherapy in patients with germ cell tumors. J Clin Oncol. 1992;10(10):1609-1614.

Abdel-Gayoum AA, El-Jenjan KB, Ghwarsha KA. Hyperlipidaemia in cisplatin-induced nephrotic rats. Hum Exp Toxicol. 1999;18(7):454-459.

Kwon SH, Ahn IS, Kim SO, Kong CS, Chung HY, Do MS, et al. Anti-obesity and hypolipidemic effects of black soybean anthocyanins. J Med Food. 2007;10(3):552-556.

Vagdatli E, Gounari E, Lazaridou E, Katsibourlia E, Tsikopoulou F, Labrianou I. Platelet distribution width: a simple, practical and specific marker of activation of coagulation. Hippokratia. 2010;14(1):28-32.

Salonen JT. Antioxidants and platelets. Ann Med. 1989;21(1):59-62.

Sobotkova A, Masova-Chrastinova L, Suttnar J, Stikarova J, Majek P, Reicheltova Z, et al. Antioxidants change platelet responses to various stimulating events. Free Radic Biol Med. 2009;47(12):1707-1714.

Pignatelli P, Di Santo S, Buchetti B, Sanguigni V, Brunelli A, Violi F. Polyphenols enhance platelet nitric oxide by inhibiting protein kinase C-dependent NADPH oxidase activation: effect on platelet recruitment. FASEB J. 2006;20(8):1082-1089.

Aydin B, Unsal M, Sekeroglu ZA, Gulbahar Y. The antioxidant and antigenotoxic effects of pycnogenol((R)) on rats treated with cisplatin. Biol Trace Elem Res. 2011;142(3):638-650.

Kohnen S, Franck T, Van Antwerpen P, Boudjeltia KZ, Mouithys-Mickalad A, Deby C, et al. Resveratrol inhibits the activity of equine neutrophil myeloperoxidase by a direct interaction with the enzyme. J Agric Food Chem. 2007;55(20):8080-8087.

Kurutas EB, Cetinkaya A, Bulbuloglu E, Kantarceken B. Effects of antioxidant therapy on leukocyte myeloperoxidase and Cu/Zn-superoxide dismutase and plasma malondialdehyde levels in experimental colitis. Mediators Inflamm. 2005;2005(6):390-394.

Yao X, Panichpisal K, Kurtzman N, Nugent K. Cisplatin nephrotoxicity: a review. Am J Med Sci. 2007;334(2):115-124.

Ramesh G, Reeves WB. TNF-alpha mediates chemokine and cytokine expression and renal injury in cisplatin nephrotoxicity. J Clin Invest. 2002;110(6):835-842.

Wedi B, Straede J, Wieland B, Kapp A. Eosinophil apoptosis is mediated by stimulators of cellular oxidative metabolisms and inhibited by antioxidants: involvement of a thiol-sensitive redox regulation in eosinophil cell death. Blood. 1999;94(7):2365-2373.

Silici S, Ekmekcioglu O, Kanbur M, Deniz K. The protective effect of royal jelly against cisplatin-induced renal oxidative stress in rats. World J Urol. 2011;29(1):127-132.

Akomolafe SF, Akinyemi AJ, Anadozie SO. Phenolic acids (gallic and tannic acids) modulate antioxidant status and cisplatin induced nephrotoxicity in rats. Int Sch Res Notices. 2014;2014: Article ID 984709, 8 pages.

Verma PK, Raina R, Sultana M, Singh M, Kumar P. Total antioxidant and oxidant status of plasma and renal tissue of cisplatin-induced nephrotoxic rats: protection by floral extracts of Calendula officinalis Linn. Ren Fail. 2016;38(1):142-150.


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