Cloning of alkaline protease gene from Bacillus subtilis 168

H Mir Mohammad Sadeghi, M Rabbani, M Naghitorabi


The aim of this study was to clone the serine alkaline protease-encoding gene from Bacillus subtilis 168. This protease, which can have many applications especially in detergent, may be industrially an important enzyme. For the amplification of the gene, PCR was performed with a pair of primers specifically designed for this purpose. Electrophoresis of the PCR product showed the expected band of 1329 bp. Restriction analysis also confirmed the integrity of the PCR product. After ligation of amplified gene into pTZ57R by the method of TA cloning, digestion with appropriate restriction enzymes confirmed the integrity of the cloned gene. Successful cloning of the protease gene from B. Subtilis could pave the way for the expression studies in a suitable host.


Cloning; Alkaline protease; Industrial enzyme; Bacillus subtilis 168

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