Optimization of alkaline phosphatase gene expression in E. coli

H Mir Mohammad Sadeghi, M Rabbani, S Fazeli

Abstract


Alkaline phosphatase is an enzyme with widespread use in research and industry such as protein labeling, dephosphorylation of nucleic acids, and enzyme based biosensors. In the present study, alkaline phosphatase gene was inserted into the pET15b vector. The recombinant DNA was then expressed using IPTG as an inducer. The expression of this enzyme was optimized by changing expression conditions such as IPTG concentration, time, and temperature. The obtained results demonstrate that with the expression system used, large quantities of active alkaline phosphatase can be produced in E. coli.

Keywords


Alkaline phosphatase; pET15b; E. coli K-12

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