The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line

Hanieh Nadi Yazdi , Farshad Mirzavi, Mahla Kazemian Kakhki, Amir R. Afshari, Seyed Hadi Mousavi , Mohammad Soukhtanloo

Abstract


Background and purpose: Aging is a dynamic and progressive loss of physiological integrity that leads to irreversible changes in cells and tissues, thereby increasing the risk of disability, disease, and death. Previous studies have provided evidence that D-galactose (D-gal) mimics the natural aging process in humans. On the other hand, it has been shown that α-lipoic acid (α-LA) acts as an anti-inflammatory and antioxidant compound. Therefore, this study aimed to investigate the protective effects of α-LA on D-gal-induced cellular senescence in SH-SY5Y neuroblastoma cells.

Experimental approach: Senescence was induced in SH-SY5Y cells by D-gal, and the protective effects of α-LA against D-gal toxicity were evaluated by the assays of β-galactosidase, reactive oxygen species (ROS), and antioxidant parameters in SH-SY5Y cells. In addition, the mRNA expression of Bax, Bcl-2, and p53 genes was evaluated using qRT-PCR.

Findings/Results: The results revealed that α-LA at the concentrations of 62.5 and 125 μM reduced the cytotoxicity and senescence caused by D-gal. α-LA also effectively reduced the ROS generation compared to the D-gal group. Treatment with α-LA significantly modulated the levels of malondialdehyde, total thiol, and superoxide dismutase activity, which were altered by D-gal. In addition, treatment with α-LA decreased the expression of Bax and p53 genes, while increasing the expression of the Bcl-2 gene.

Conclusion and implications: Overall, the results showed that α-LA could moderate the toxic effects of D-gal by increasing the antioxidant capacity and modulating the genes involved in apoptosis, and it deserves further studies.

 

 


Keywords


α-lipoic acid; Cellular senescence; D-galactose; Oxidative stress; SH-SY5Y neuroblastoma cells.

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