The brown alga Padina pavonica methanol and hexane partitions prevented depressive behavior induced by dexamethasone in mice

Azadeh Mesripour , Negar Asgari, Afsaneh Yegdaneh

Abstract


Background and purpose: Padina pavonica, a brown alga, displays protection against oxidative stress, neuroinflammation, and neurodegenerative disorders. Considering the beneficial effects of P. pavonica and since its antidepressant effects have not yet been studied, we investigated its methanol and hexane partitions (PMP and PHP) in mice model.

Experimental approach: In male mice (25 ± 2 g), depression was initiated by administrating dexamethasone (15 µg/kg) subcutaneously. PMP or PHP (80-160 mg/kg) was administered intraperitoneally. All the injections continued for two weeks. After the locomotor test, different depression criteria were evaluated by forced swim test (FST), marble burying test (MBT), sucrose preference (SP) test, and novelty-suppressed feeding test (NSFT).

Findings/Results: PMP (160 mg/kg) showed antidepressant effects, immobility time decreased significantly during FST (84.6 ± 10.4 s) compared to the control, food intake increased significantly during NSFT (21.1 ± 2.5 mg/g) versus the control, and SP was 81%. PHP (80 mg/kg) reduced immobility time to 124.5 ± 6.7 s compared to the vehicle and increased the SP to 85%. Dexamethasone-induced depression. While co-treatment with PMP or PHP prevented depression initiated by dexamethasone. Only PHP reduced the number of buried marbles after 30 min against the control. The applied doses did not cause significant changes in the locomotor activity.

Conclusion and implications: PMP and PHP exhibited antidepressant-like effects in mice. PHP also reduced the number of buried marbles, implying a reduction in obsessive-like behavior. These observations underscore the constituents inherent in PMP and PHP, which merit further exploration to elucidate their potential therapeutic applications.

 

 


Keywords


Animal research; Brown algae; Depression; Padina pavonica.

Full Text:

PDF

References


Gelenberg AJ. The prevalence and impact of depression. J Clin Psychiatry. 2010;71(3):e06, 26446.

DOI: 10.4088/JCP.8001tx17c.

Nutt DJ. Relationship of neurotransmitters to the symptoms of major depressive disorder. J Clin Psychiatry. 2008;69(Suppl E1):4-7.PMID: 18494537.

Lindqvist D, Dhabhar FS, James SJ, Hough CM, Jain FA, Bersani FS, et al. Oxidative stress, inflammation and treatment response in major depression. Psychoneuroendocrinology. 2017;76:197-205.DOI: 10.1016/j.psyneuen.2016.11.031.

Safaeian L, Hajhashemi V, Haghjoo Javanmard S, Sanaye Naderia H. The effect of protocatechuic acid on blood pressure and oxidative stress in glucocorticoid-induced hypertension in rat. Iran J Pharm Res. 2016;15:83-91.PMID: 28228807.

Mesripour A, Gholamzadeh E, Yegdaneh A. Extraction and fractionation of the seaweed Sargassum plagyophylum and evaluation of fractions on depression induced by interferon alpha in mice. Adv Biomed Res. 2022;11:59,1-6.DOI: 10.4103/abr.abr_186_21.

Hannan MA, Dash R, Haque MN, Mohibbullah M, Sohag AAM, Rahman MA, et al. Neuroprotective potentials of marine algae and their bioactive metabolites: pharmacological insights and therapeutic advances. Mar Drugs. 2020;18(7):347,1-50.DOI: 10.3390/md18070347.

Mesripour A, Rabian N, Yegdaneh A. The effect of different partitions of seaweed Sargassum plagyophylum on depression behavior in mice model of despair. J Complement Integr Med. 2019;16(4):1-7.DOI: 10.1515/jcim-2018-0207.

Generalić Mekinić I, Skroza D, Šimat V, Hamed I, Čagalj M, Popović Perković Z. Phenolic content of brown algae (Pheophyceae) species: extraction, identification, and quantification. Biomolecules. 2019;9(6):244,1-25.DOI: 10.3390/biom9060244.

Yegdaneh A, Mesripour A, Iravani M. The brown Alga Padina australis total extract and depressive-like behavior following BCG inoculation in mice. Pharm Biomed Res. 2023;9(2):125-132.DOI: 10.32598/PBR.9.2.1116.1.

Silberfeld T, Bittner L, Fernández-García C, Cruaud C, Rousseau F, de Reviers B, et al. Species diversity, phylogeny and large scale biogeographic patterns of the genus Padina (Phaeophyceae, Dictyotales). J Phycol. 2013;49(1):130-142.DOI: 10.1111/jpy.12027.

Hakim MM, Patel IC. A review on phytoconstituents of marine brown algae. Futur J Pharm Sci. 2020; 6: 129,1-11.DOI: 10.1186/s43094-020-00147-6.

Shanmuganathan B, Sheeja Malar D, Sathya S, Pandima Devi K. Antiaggregation potential of Padina gymnospora against the toxic Alzheimer’s beta-amyloid peptide 25-35 and cholinesterase inhibitory property of its bioactive compounds. PLoS One. 2015;10(11):e0141708,1-14.DOI: 10.1371/journal.pone.0141708.

Generalić Mekinić I, Šimat V, Botić V, Crnjac A, Smoljo M, Soldo B, et al. Bioactive phenolic metabolites from adriatic brown algae Dictyota dichotoma and Padina pavonica (Dictyotaceae). Foods. 2021;10(6):1187,1-10.DOI: 10.3390/foods10061187.

Shafay SE, El-Sheekh M, Bases E, El-Shenody R. Antioxidant, antidiabetic, anti-inflammatory and anticancer potential of some seaweed extracts. Food Sci Technol. 2021;42: e20521,1-12.DOI: 10.1590/fst.20521.

Mesripour A, Alhimma F, Hajhashemi V. The effect of vitamin B6 on dexamethasone-induced depression in mice model of despair. Nutr Neurosci. 2019;22(10):744-749.DOI: 10.1080/1028415X.2018.1442184.

Blasco-Serra A, González-Soler EM, Cervera-Ferri A, Teruel-Martí V, Valverde-Navarro AA. A standardization of the novelty-suppressed feeding test protocol in rats. Neurosci Lett. 2017;658:73-78.DOI: 10.1016/j.neulet.2017.08.019.

Mesripour A, Golbidi M, Hajhashemi V. Dextromethorphan improved cyclosporine-induced depression in mice model of despair. Res Pharm Sci. 2020;15(5):447-453.DOI: 10.4103/1735-5362.297847.

Mesripour A, Rakhshankhah P. A synbiotic mixture ameliorates depressive behavior induced by dexamethasone or water avoidance stress in a mouse model. Turk J Pharm Sci. 2021;18(1):21-27.DOI: 10.4274/tjps.galenos.2019.71300.

Mesripour A, Golchin S. Vitamin B6 antidepressant effects are comparable to common antidepressant drugs in Bacillus-Calmette-Guerin induced depression model in mice. Iran J Psychiatry. 2022;17(2):208-216.DOI: 10.18502/ijps.v17i2.8911.

Deacon RMJ. Digging and marble burying in mice: simple methods for in vivo identification of biological impacts. Nat Protoc. 2006;1(1):122-124.DOI: 10.1038/nprot.2006.20.

Attard E. A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols. Open Life Sci. 2013;8(1): 48-53.DOI: 10.2478/s11535-012-0107-3.

Planchez B, Surget A, Belzung C. Animal models of major depression: drawbacks and challenges. J Neural Transm. 2019;126:1383-1408.DOI: 10.1007/s00702-019-02084-y.

Dulawa SC, Hen R. Recent advances in animal models of chronic antidepressant effects: the novelty-induced hypophagia test. Neurosci Biobehav Rev. 2005;29(4-5):771-783.DOI: 10.1016/j.neubiorev.2005.03.017.

Iijima M, Fukumoto K, Chaki S. Acute and sustained effects of a metabotropic glutamate 5 receptor antagonist in the novelty-suppressed feeding test. Behav Brain Res. 2012;235(2):287-292.DOI: 10.1016/j.bbr.2012.08.016.

Numakawa T, Odaka H, Adachi N. Actions of brain-derived neurotrophic factor and glucocorticoid stress in neurogenesis. Int J Mol Sci. 2017;18(11):2312,1-15.DOI: 10.3390/ijms18112312.

Steckler T, Holsboer F, Reul JM. Glucocorticoids and depression. Best Pract Res Clin Endocrinol Metab. 1999;13(4):597-614.DOI: 10.1053/beem.1999.0046.

Schepers M, Martens N, Tiane A, Vanbrabant K, Liu HB, Lütjohann D, et al. Edible seaweed-derived constituents: an undisclosed source of neuroprotective compounds. Neural Regen Res. 2020;15(5):790-795.DOI: 10.4103/1673-5374.268894.

Mesripour A, Shahnooshi S, Hajhashemi V. Celecoxib, ibuprofen, and indomethacin alleviate depression-like behavior induced by interferon-alfa in mice. J Complement Integr Med. 2019;17(1):20190016,1-9.DOI: 10.1515/jcim-2019-0016.

Heo SJ, Ko SC, Kang SM, Kang HS, Kim JP, Kim SH, et al. Cytoprotective effect of fucoxanthin isolated from brown algae Sargassum siliquastrum against H2O2-induced cell damage. Eur Food Res Technol. 2008;228(1):145-151.DOI: 10.1007/s00217-008-0918-7.

Santos-Sánchez NF, Salas-Coronado R, Villanueva-Cañongo C, Hernández-Carlos B. Antioxidant compounds and their antioxidant mechanism. Antioxidants. 2019;10:1-30.DOI: 10.5772/intechopen.85270.

Wang H, Jin M, Xie M, Yang Y, Xue F, Li W, et al. Protective role of antioxidant supplementation for depression and anxiety:a meta-analysis of randomized clinical trials. J Affect Disord. 2023; 323:264-279.DOI: 10.1016/j.jad.2022.11.072.

Liu XY, Liu D, Lin GP, Wu YJ, Gao LY, Ai C, et al. Anti-ageing and antioxidant effects of sulfate oligosaccharides from green algae Ulva lactuca and Enteromorpha prolifera in SAMP8 mice. Int J Biol Macromol. 2019;139:342-351.DOI: 10.1016/j.ijbiomac.2019.07.195.

Park JH, Ahn JH, Lee TK, Park CW, Kim B, Lee JC, et al. Laminarin pretreatment provides neuroprotection against forebrain ischemia/reperfusion injury by reducing oxidative stress and neuroinflammation in aged gerbils. Mar Drugs. 2020;18(4):213,1-14.DOI: 10.3390/md18040213.

Hayes M. Seaweeds: a nutraceutical and health food. In: Seaweed Sustainability. Academic Press. 2015. pp. 365-387.DOI: 10.1016/B978-0-12-418697-2.00014-3.

Thurfah JN, Christine, Bagaskhara PP, Alfian SD, Puspitasari IM. Dietary supplementations and depression. J Multidiscip Healthc. 2022;15:1121-1141.DOI: 10.2147/JMDH.S360029.

Aliomrani M, Mesripour A, Saleki Mehrjardi A. Creatine and alpha-lipoic acid antidepressant-like effect following cyclosporine A administration. Turk J Pharm Sci. 2022;19(2):196-201.DOI: 10.4274/tjps.galenos.2021.27217.

Mesripour A, Payandekhah F. The noradrenergic system is partly involved in resveratrol antidepressant and anti-obsessive-like effects in mice model. Res J Pharmacogn. 2021;8(3):67-75.DOI: 10.22127/RJP.2021.277101.1686.

Sugimoto Y, Tagawa N, Kobayashi Y, Hotta Y, Yamada J. Effects of the serotonin and noradrenaline reuptake inhibitor (SNRI) milnacipran on marble burying behavior in mice. Biol Pharm Bull. 2007;30(12):2399-2401.DOI: 10.1248/bpb.30.2399.


Refbacks

  • There are currently no refbacks.


Creative Commons LicenseThis work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.