Isolation and identification of 6-methoxy parillin and coniferin from the bulbs of Allium affine L.

Narges Vakili , Mahsa Esmaeilifallah, Masoud Sadeghi Dinani


Background and purpose: Phytochemically, Allium species are a rich source of important secondary metabolites especially steroidal saponin and sapogenins, flavonoids, and sulfur compounds. As a member of this genus, Allium affine, which is locally known as “tareh kouhi”, is an endemic plant of middle Asian countries.

Experimental approach: Bulbs of A. affine were collected and air-dried in the shade. The chloroform-methanol (9:1) extract of the sample was subjected to purification by MPLC and HPLC. Structure elucidation of isolated compounds was done using comprehensive spectroscopic methods including 1D-NMR, 2D-NMR, and MS.

Findings/Results: A steroidal saponin structurally related to parillin and a phenylpropanoid glycoside (coniferin) were isolated and identified from the plant chloroform-methanol extract.

Conclusion and implication: To the best of our knowledge isolation of these potentially medicinal compounds from A. affine was reported for the first time in this study.




Allium affine Ledeb, Structure Elucidation, Steroidal Saponin, Phenylpropanoid.

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