Naringenin potentiated β-sitosterol healing effect on the scratch wound assay

Manal Mohammad Abbas , Naseer Al-Rawi, Manal Ahmad Abbas , Iqbal Al-Khateeb

Abstract


In the present investigation scratch wound assay was used to study the ability of several combinations of each flavonoid (chrysin, naringenin or resveratrol) with β-sitosterol to heal wounds in vitro. MTT test was performed to determine if the combination of flavonoid with β-sitosterol was toxic to fibroblasts or not. Also, superoxide dismutase (SOD) activity and interleukin-1β (IL-1β) concentrations were measured.The best closure rates were obtained with β-sitosterol combined with naringenin and β-sitosterol combined with resveratrol. The combination that produced the best closure rate namely β-sitosterol with naringenin increased SOD activity significantly. However, this combination was not better than naringenin or  β-sitosterol alone in reducing IL-1β concentration. The results of MTT test indicated that the combination as well as β-sitosterol alone or naringenin alone has no toxic effect on fibroblasts. In conclusion, the combination of β-sitosterol and naringenin exerted a synergistic effect on wound closure without decreasing the viability of fibroblasts, increased antioxidant defense mechanism and decreased IL-1β.


Keywords


Beta-sitosterol; Interleukin-1β; Naringenin; Scratch wound assay; SOD; Synergism.

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References


Hakim N, editor. Artificial Organs. In: Andersson LC, Nettelblad H, Kratz G. From Basic Wound Healing to Modern Skin Engineering. London: Springer; 2009. pp. 93-105.

Cukjati D, Reberšek S, Miklavčič D. A reliable method of determining wound healing rate. Med Biol Eng Comput. 2001;39(2):263-271.

Toriseva M, Kähäri VM. Proteinases in cutaneous wound healing. Cell Mol Life Sci. 2009;66(2):203-224.

Diegelmann RF. From the selected works of Robert F. Diegelmann Ph.D. Front Biosci. 2004;9:283-289.

Hu Y, Liang D, Li X, Liu HH, Zhang X, Zheng M, et al. The role of interleukin-1 in wound biology. Part I: Murine in silico and in vitro experimental analysis. Anesth Analg. 2010;111(6):1525-1533.

Ansell DM, Holden KA, Hardman MJ. Animal models of wound repair: Are they cutting it? Exp Dermatol. 2012;21(8):581-585.

Anlas C, Bakirel T, Ustun-Alkan F, Celik B, Baran MY, Ustuner O, et al. In vitro evaluation of the therapeutic potential of Anatolian kermes oak (Quercus coccifera L.) as an alternative wound healing agent. Ind Crop Prod. 2019;137:24-32.

Davis SC, Perez R. Cosmeceuticals and natural products: wound healing. Clin Dermatol. 2009;27(5):502-506.

Kooti W, Ghasemiboroon AAM, Harizi M, Afzalzadeh M, Afshar M, Hozeyli S, et al. Effect of compound cream, a mixture of honey, fish oil, Hypericum perforatum L. and Achilea mille folium L. on full thickness skin wound in rat. Res Pharm Sci. 2012;7(5):18.

Yaman I, Derici H, Kara C, Kamer E, Diniz G, Ortac R, et al. Effects of resveratrol on incisional wound healing in rats. Surg Today. 2013;43(12):1433-1438.

Deldar Y, Pilehvar-Soltanahmadi Y, Dadashpour M, Montazer Saheb S, Rahmati-Yamchi M, Zarghami N. An in vitro examination of the antioxidant, cytoprotective and anti-inflammatory properties of chrysin-loaded nanofibrous mats for potential wound healing applications. Artif Cells Nanomed Biotechnol. 2018;46(4):706-716.

Mir IA, Tiku AB. Chemopreventive and therapeutic potential of “naringenin,” a flavanone present in citrus fruits. Nutr Cancer. 2015;67(1):27-42.

Bin Sayeed MS, Karim SMR, Sharmin T, Morshed MM. Critical analysis on characterization, systemic effect, and therapeutic potential of beta-sitosterol: a plant-derived orphan phytosterol. Medicines (Basel). 2016;3(4). pii: E29.

Al-Roujayee AS. Naringenin improves the healing process of thermally-induced skin damage in rats. J Int Med Res. 2017;45(2):570-582.

Tsala DE, Amadou D, Habtemariam S. Natural wound healing and bioactive natural products. Phytopharmacology. 2013;4(3):532-560.

Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. Ethnopharmacological approaches to wound healing-exploring medicinal plants of India. J Ethnopharmacol. 2007;114(2):103-113.

Liang CC, Park AY, Guan JL. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc. 2007;2(2):329-333.

Abbas MM, Abbas MA, Kandil YI. Cytotoxic activity of Varthemia iphionoides essential oil against various human cancer cell lines. Acta Pol Pharm. 2019;76(4):701-706.

Theoret C, editor. Equine Wound Management. In: Theoret C, Physiology of wound healing. 3rd ed. Wiley & Sons, Inc; 2016. pp. 1-13.

Shahi Z, Nakhaee M, Hasani M, Balandeh A. Effect of the powder and hydroalcoholic extract of the fruit sheath of Prosopis farcta on the fibroblasts and angiogenic process in wound healing of rat.Res Pharm Sci. 2012;7(5):S820.

Stompor M, Uram Ł, Podgórski R. In vitro effect of 8-prenylnaringenin and naringenin on fibroblasts and glioblastoma cells-cellular accumulation and cytotoxicity. Molecules. 2017;22(7). pii: E1092.

Kandhare AD, Alam J, Patil MV, Sinha A, Bodhankar SL. Wound healing potential of naringin ointment formulation via regulating the expression of inflammatory, apoptotic and growth mediators in experimental rats. Pharm Biol. 2016;54(3):419-432.

Stipcevic T, Piljac J, Berghe DV. Effect of different flavonoids on collagen synthesis in human fibroblasts. Plant Foods For Hum Nutr. 2006;61(1):29-34.

Lee ES, Shin MO, Yoon S, Moon JO. Resveratrol inhibits dimethylnitrosamine-induced hepatic fibrosis in rats. Arch Pharm Res. 2010;33(6):925-932.

Sardari K, Pedram S, Zojaji V, Maleki M, Mohri M, Dehgan M, et al. Effects of Zn-7® on open wound healing in dogs. Comp Clin Path. 2006;15(4):237-243.

Thiem B, Goślińska O. Antimicrobial activity of Rubus chamaemorus leaves. Fitoterapia. 2004;75(1):93-95.

Alvarez-Suarez JM, Giampieri F, Cordero M, Gasparrini M, Forbes-Hernández TY, Mazzoni L,et al. Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing. J Funct Foods. 2016;25:38-49.

Chamberlain CS, Leiferman EM, Frisch KE, Duenwald-Kuehl SE, Brickson SL, Murphy WL,et al. Interleukin-1 receptor antagonist modulates inflammation and scarring after ligament injury. Connect Tissue Res. 2014;55(3):177-186.

Ren K, Torres R. Role of interleukin-1beta during pain and inflammation. Brain Res Rev. 2009;60(1):57-64.

Mia MM, Boersema M, Bank RA. Interleukin-1β attenuates myofibroblast formation and extracellular matrix production in dermal and lung fibroblasts exposed to transforming growth factor-β1. PloS One. 2014;9(3):e91559,1-19.


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