The protective effect of sodium benzoate on aluminum toxicity in PC12 cell line

Rita Arabsolghar, Jamileh Saberzadeh, Forouzan Khodaei, Rozhin Abbasi Borojeni, Marjan Khorsand, Marzieh Rashedinia

Abstract


Sodium benzoate (SB) is one of the food additives and preservatives that prevent the growth of fungi and bacteria. SB has been shown to improve the symptoms of neurodegenerative disease such as Alzheimer’s disease. The aim of this study was to evaluate the effect of SB on the cell survival and cellular antioxidant indices after exposure to aluminum maltolate (Almal) in PC12 cell line as a model of neurotoxicity. The cells exposed to different concentrations of SB (0.125 to 3 mg/mL) in the presence of Almal (500 µM) and cell viability, the level of reactive oxygen species (ROS), glutathione content and catalase activity were measured. The results showed that low concentrations of SB caused an increase in the cell survival, but cell viability was reduced in high concentrations. SB could  neither prevent the level of ROS production nor change glutathione content. SB (0.5 mg/mL) significantly increased the catalase enzyme activity as compared to the Almal. This study suggested that SB did not completely protect the cell to aluminum-induced free radicals toxicity. Possibly SB improves the symptoms of neurodegenerative disease by other mechanisms.


Keywords


Sodium benzoate; Aluminum; Neurotoxicity; PC12; Oxidative stress; Glutathione

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References


Kumar V, Gill KD. Aluminium neurotoxicity: neurobehavioural and oxidative aspects. Arch Toxicol 2009; 83:965-978.

Amjad S, Umesalma S. Protective effect of Centella asiatica against aluminium-induced neurotoxicity in cerebral cortex, striatum, hypothalamus and hippocampus of rat brain-histopathological, and biochemical approach. J Mol Biomark Diagn. 2015;2015:212.

Savory J, Herman MM, Ghribi O. Mechanisms of aluminum-induced neurodegeneration in animals: Implications for Alzheimer's disease. J Alzheimers Dis. 2006;10:135-144.

Krewski D, Yokel RA, Nieboer E, Borchelt D, Cohen J, Harry J, et al. Human health risk assessment for aluminium, aluminium oxide, and aluminium hydroxide. J Toxicol Environ Health B Crit Rev. 2007;10:1-269.

Cannon JR, Greenamyre JT. The role of environmental exposures in neurodegeneration and neurodegenerative diseases. Toxicol Sci. 2011;124(2):225-250..

Zeng K-W, Fu H, Liu G-X, Wang X-M. Aluminum maltolate induces primary rat astrocyte apoptosis via overactivation of the class III PI3K/Beclin 1-dependent autophagy signal. Toxicol In Vitro. 2012; 26:215-220.

Walton J. Evidence for participation of aluminum in neurofibrillary tangle formation and growth in Alzheimer's disease. J Alzheimers Dis. 2010; 22:65-72.

Nair B. Final report on the safety assessment of benzyl alcohol, benzoic acid, and sodium benzoate. Int J Toxicol. 2001;20:23-50.

Reddy MV, Aruna G, Parameswari SA, Banu BH, Reddy PJ. Estimated daily intake and exposure of sodium benzoate and potassium sorbate through food products in school children of tirupati, india. Int J Pharm Pharm Sci. 2015;7:129-133.

Heydaryinia A, Veissi M, Sadadi A. A comparative study of the effects of the two preservatives, sodium benzoate and potassium sorbate on Aspergillus niger and Penicillium notatum. Jundishapur J Microbiol .2011;4.

Gropman A, Summar M, Leonard J. Neurological implications of urea cycle disorders. J Inherit Metab Dis. 2007;30:865-879.

Jana A, Modi KK, Roy A, Anderson JA, van Breemen RB, Pahan K. Up-regulation of neurotrophic factors by cinnamon and its metabolite sodium benzoate: therapeutic implications for neurodegenerative disorders. J Neuroimmune Pharmacol. 2013;8:739-755.

Modi KK, Roy A, Brahmachari S, Rangasamy SB, Pahan K. Cinnamon and its metabolite sodium benzoate attenuate the activation of p21 rac and protect memory and learning in an animal model of Alzheimer’s disease. PloS one. 2015;10:e0130398.

Yadav A, Kumar A, Das M, Tripathi A. Sodium benzoate, a food preservative, affects the functional and activation status of splenocytes at non cytotoxic dose. Food Chem Toxicol. 2016;88:40-47.

Lin C-H, Chen P-K, Chang Y-C, Chuo L-J, Chen Y-S, Tsai GE, et al. Benzoate, a D-amino acid oxidase inhibitor, for the treatment of early-phase Alzheimer disease: a randomized, double-blind, placebo-controlled trial. Biol Psychiatry. 2014;75:678-685.

Bertholf RL, Herman MM, Savory J, Carpenter RM, Sturgill BC, Katsetos CD, et al. A long-term intravenous model of aluminum maltol toxicity in rabbits: tissue distribution, hepatic, renal, and neuronal cytoskeletal changes associated with systemic exposure. Toxicol Appl Pharmacol. 1989;98:58-74.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63.

Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem. 1969;27:502-522.

Zal F, Mostafavi Pour Z, Vessal M. Comparison of the effects of vitamin e and/or quercetin in attenuating chronic cyclosporine a induced nephrotoxicity in male rats. Clin Expe Pharmacol Physiol. 2007;34:720-724.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-254.

Satoh E, Okada M, Takadera T, Ohyashiki T. Glutathione depletion promotes aluminum-mediated cell death of PC12 cells. Biol Pharm Bull. 2005;28:941-946.

Saberzadeh J, Arabsolghar R, Takhshid MA. Alpha synuclein protein is involved in Aluminum-induced cell death and oxidative stress in PC12 cells. Brain Res. 2016;1635:153-160.

Ohyashiki T, Satoh E, Okada M, Takadera T, Sahara M. Nerve growth factor protects against aluminum-mediated cell death. Toxicology. 2002;176:195-207.

Johnson VJ, Kim SH, Sharma RP. Aluminum-maltolate induces apoptosis and necrosis in neuro-2a cells: potential role for p53 signaling. Toxicol Sci. 2005;83:329-339.

Griffioen KJ, Ghribi O, Fox N, Savory J, DeWitt DA. Aluminum maltolate-induced toxicity in NT2 cells occurs through apoptosis and includes cytochrome c release. Neurotoxicology. 2004;25:859-867.

Satoh E, Okada M, Takadera T, Ohyashiki T. Glutathione depletion promotes aluminum-mediated cell death of PC12 cells. Biol Pharm Bull. 2005;28:941-946.

Cervantes A, Pinedo HM, Lankelma J, Schuurhuis GJ. The role of oxygen-derived free radicals in the cytotoxicity of doxorubicin in multidrug resistant and sensitive human ovarian cancer cells. Cancer Lett. 1988;41(2):169-177.

Tripathi BN, Gaur JP. Relationship between copper- and zinc-induced oxidative stress and proline accumulation in Scenedesmus sp. Planta. 2004;219:397-404.

Hu M, Wang J, Cai J, Wu Y, Wang X. [Analysis of sodium benzoate biotoxicity by atomic force microscope]. Sheng Wu Gong Cheng Xue Bao. 2008;24:1428-1432.

Murakami K, Yoshino M. Aluminum decreases the glutathione regeneration by the inhibition of NADP-isocitrate dehydrogenase in mitochondria. J Cell Biochem .2004;93:1267-1271.

Silva-Adaya D, Gonsebatt ME, Guevara J. Thioredoxin system regulation in the central nervous system: experimental models and clinical evidence. Oxid Med Cell Longev. 2014;2014:590808.

Mohammadirad A, Abdollahi M. A systematic review on oxidant/antioxidant imbalance in aluminium toxicity. Int J Pharmacol. 2011;7:12-21.

Anane R, Creppy EE. Lipid peroxidation as pathway of aluminium cytotoxicity in human skin fibroblast cultures: prevention by superoxide dismutase+catalase and vitamins E and C. Hum Exp Toxicol. 2001;20:477-481.

Swain C, Chainy GB. Aluminum effect on lipid peroxidation and on the activities of superoxide dismutase and catalase in the cerebral hemisphere and liver of young chicks. J Trace Elem Med Biol. 1997;11:77-82.

Lushchak VI. Classification of oxidative stress based on its intensity. EXCLI J. 2014;13:922.

Esposito S, Pristera A, Maresca G, Cavallaro S, Felsani A, Florenzano F, et al. Contribution of serine racemase/D-serine pathway to neuronal apoptosis. Aging Cell. 2012;11:588-598.

Lane H-Y, Lin C-H, Green MF, Hellemann G, Huang C-C, Chen P-W, et al. Add-on treatment of benzoate for schizophrenia: a randomized, double-blind, placebo-controlled trial of D-amino acid oxidase inhibitor. JAMA Psychiatry. 2013;70:1267-1275.

Noorafshan A, Erfanizadeh M, Karbalay-Doust S. Sodium benzoate, a food preservative, induces anxiety and motor impairment in rats. Neurosciences (Riyadh). 2014;19:24-28.

Kilic N, Balkan E, Akgoz S, Sen N, Dogruyol H. Comparison of the effectiveness and side-effects of tolterodine and oxybutynin in children with detrusor instability. Int J Urol. 2006;13:105-108.

Arnold LE, Lofthouse N, Hurt E. Artificial food colors and attention-deficit/hyperactivity symptoms: conclusions to dye for. Neurotherapeutics. 2012;9:599-609.

Beezhold BL, Johnston CS, Nochta KA. Sodium benzoate–rich beverage consumption is associated with increased reporting of ADHD symptoms in college students a pilot investigation. J Atten Disord. 2014;18:236-241.


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