Evaluation of fibrinolytic and antioxidant effects of Allium affine hydroalcoholic extract

Masoud Sadeghi, Leila Safaeian, Mohammadreza Aghaye Ghazvini, Mojtaba Ramezani


Allium affine is a member of Amaryllidaceae family, which grows wildly in some western regions of Iran. Limited information is available about the pharmacological activities of this plant. The present study aimed to evaluate the fibrinolytic and antioxidant effects of hydroalcoholic extract of A. affine aerial parts. The in vitro antioxidant properties of the extract were evaluated by total phenolic content assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and ferric reducing antioxidant power (FRAP) assay. The in vivo studies included the determination of hydroperoxides level and FRAP value in serum samples of rats receiving i.p. injections of the plant extract for 21 days. The fibrinolytic activity of the extract was quantitatively evaluated by measuring the clot weight. In vitro antioxidant analysis exhibited the promising potential of DPPH scavenging and total antioxidant capacity of A. affine extract. In the in vivo analysis, A. affine extract reduced the serum hydroperoxides level and increased the serum total antioxidant capacity in rats. In vitro fibrinolytic assay also elucidated notable thrombolytic activity of the plant extract. The results of this study revealed the valuable antioxidant and in vitro fibrinolytic activities of A. affine extract. Further studies are needed for better evaluation of anticoagulant and thrombolytic activities of this plant and understanding its detailed mechanisms. 


Allium affine; Antioxidant; Fibrinolytic agents

Full Text:



Hosseinzadeh S, Jafarikukhdan A, Hosseini A, Armand R. The application of medicinal plants in traditional and modern medicine: A review of Thymus vulgaris. Int J Clin Med. 2015;6: 635-642.

van Andel T, Carvalheiro LG. Why urban citizens in developing countries use traditional medicines: the case of suriname. Evid Based Complement Alternat Med. 2013;2013:687197.

Neshati F, Fritsch RM. Seed characters and testa sculptures of some Iranian Allium L. species (Alliaceae). Feddes Repert. 2009;120:322-332.

Petrovska BB, Cekovska S. Extracts from the history and medical properties of garlic. Pharmacogn Rev. 2010;4:106-110.

Bisen PS, Emerald M. Nutritional and therapeutic potential of garlic and onion (Allium sp.). Curr Nutr Food Sci. 2016;12:190-199.

Rahman K, Lowe GM. Garlic and cardiovascular disease: A critical review. J Nutr. 2006;136(3 Suppl):736S-740S.

Komarov VL. Flora U.R.S.S. 4th Vol. Moscow and Leningrad: Academiae Scientiarum U.R.S.S.; 1935. pp. 239-240.

Gregory M, Fritsch RM, Friesen NW, Khassanov FO, McNeal DW. Nomenclator alliorum. Allium names and synonyms - a World guide. Royal Botanic Gardens, Kew; 1998. pp. 18-19.

Namin HH, Mehrvarz SS, Zarre S. Scape anatomy of Allium (Alliaceae) in Iran and its systematic application. Thaiszia J Bot. 2009;19:37-45.

Sobolewska D, Michalska K, Podolak I, Grabowska K. Steroidal saponins from the genus Allium. Phytochem Rev. 2016;15:1-35.

Waller G, Yamasaki K. Saponins used in traditional and modern medicine. NewYork: Planum Press; 1996. P. 525.

Tian Y, Ma S, Lin B, Kou J, Yu B. Anti-thrombotic activity of DT-13, a saponin isolated from the root tuber of Liriope muscari. Indian J Pharmacol. 2013;45(45):283-285.

Zolfaghari B, Shokoohinia Y, Ramezanlou P, Sadeghi A, Mahmoudzadeh M, Minaiyan M. Effects of methanolic and butanolic fractions of Allium elburzense Wendelbo bulbs on blood glucose level of normal and STZ-induced diabetic rats. Res Pharm Sci. 2012;7(4):201-207.

Jastrzebski Z, Leontowicz H, Leontowicz M, Namiesnik J, Zachwieja Z, Barton H, et al. The bioactivity of processed garlic (Allium sativum L.) as shown in vitro and in vivo studies on rats. Food Chem Toxicol. 2007;45:1626-1633.

Safaeian L, Ghannadi A, Javanmard SH, Vahidian MH. The effects of hydroalcoholic extract of Ferula foetida stems on blood pressure and oxidative stress in dexamethasone-induced hypertensive rats. Res Pharm Sci. 2015;10(4):326-334.

Safaeian L, Ghasemi-Dehkordi N, Javanmard ShH, Namvar H. Antihypertensive and antioxidant effects of a hydroalcoholic extract obtained from aerial parts of Otostegia persica (Burm.) Boiss. Res Pharm Sci. 2015;10:192-199.

Ali R, Hossain M, Runa JF, Hasanuzzaman M, Islam M. Evaluation of thrombolytic potential of three medicinal plants available in Bangladesh, as a potent source of thrombolytic compounds. Avicenna J Phytomed. 2014;4:430-436.

Yin MC, Cheng WS. Antioxidant activity of several Allium members. J Agric Food Chem. 1998;46:4097-4101.

Borek C. Antioxidant health effects of aged garlic extract. J Nutr. 2001;131:1010-1015.

Benkeblia N. Free-radical scavenging capacity and antioxidant properties of some selected onions (Allium cepa L.) and garlic (Allium sativum L.) extracts. Braz Arch Boil Technol. 2005;48:5-9.

Ben Arfa A, Najjaa H, Yahia B, Tlig A, Neffati M. Antioxidant capacity and phenolic composition as a function of genetic diversity of wild Tunisian leek (Allium ampeloprasum L.). Acad J Biotechnol. 2015;3:15-26.

Chen S, Shen X, Cheng S, Li P, Du J, Chang X, et al. Evaluation of garlic cultivars for polyphenolic content and antioxidant properties. PLoS One. 2013;8(11):e79730.

Makheja AN, Bailey JM. Antiplatelet constituents of garlic and onion. Inflamm Res. 1990; 29:360-363.

Zhang R, Huang B, Du D, Guo X, Xin G, Xing Z, et al. Anti-thrombosis effect of diosgenyl saponins in

vitro and in vivo. Steroids. 2013;78:1064-1070.

Kou J, Tian Y, Tang Y, Yan J, Yu B. Antithrombotic activities of aqueous extract from Radix Ophiopogon japonicus and its two constituents. Biol Pharm Bull. 2006;29:1267-1270.

Kimura Y, Okuda H. Effects of flavonoids isolated from Scutellariae radix on fibrinolytic system induced by trypsin in human umbilical vein endothelial cells. J Nat Prod. 1997;60:598-601.

Rajput MS, Mathur V, Agrawal P, Chandrawanshi HK, Pilaniya U. Fibrinolytic activity of kaempferol isolated from the fruits of Lagenaria siceraria (Molina) Standley. Nat Prod Res. 2011;25:1870-1875.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons LicenseThis work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.