Antibacterial and anti-inflammatory activities of marine Brevibacterium sp.

Vennam Srilekha, Gudikandula Krishna, Vutukuru Seshasrinivas, Maringanti Alha Singara Charya

Abstract


The marine environment covers three quarters of the surface of the planet and is estimated to be home to more than 80% of life but yet it remains largely unexplored. It harbours a number of macro and microorganisms that have developed unique metabolic abilities to ensure their survival in diverse and hostile habitats, resulting in the biosynthesis of an array of secondary metabolites with specific activities. In this study, pigment forming bacterial strains were isolated from the sea surface inter tidal zones at different sampling sites along the Visakhapatnam coastal region. The bacterial isolates showed various types of colour pigments like pink, yellow, orange, and brown. Out of 26 pigmented isolates obtained, the bacterial isolate with bright yellow pigmentation was selected for further study. This strain was identified as Brevibacterium sp by using morphological, physiological, biochemical and 16s rRNA sequencing methods. The pigment was extracted in methanol solvent and antibacterial activity of the pigments extracted from the bacteria was determined and it was found active against pathogenic bacteria. The pigment extract was tested In vivo for anti-inflammatory activity and was effective.


Keywords


Pigments; Antibacterial; Brevibacterium sp.; Anti-inflammatory activity

Full Text:

PDF

References


Fenical W, Jensen PR. Marine microorganisms: a new biomedical resource. In Pharmaceutical and Bioactive Natural Products. Springer US. 1993; 419-457.

Fenical W. Chemical studies of marine bacteria: developing a new resource. Chemical Reviews. 1993;5:1673-1683.

Kobayashi E, Nakano H, Morimoto M, Tamaoki T. Calphostin C (UCN-1028C), a novel microbial compound, is a highly potent and specific inhibitor of protein kinase C. Biochem Biophys Res Commun. 1989;159:548-553.

McConnell OJ, Longley RE, Koehn FE. The discovery of marine natural products with therapeutic potential. Biotechnology. 1994;26:109-174.

Mortensen A. Carotenoids and other pigments as natural colorants. Pure and Applied Chemistry. 2006;78:1477-1491.

Venil CK, Zakaria ZA, Ahmad WA. Bacterial pigments and their applications. Process Biochemistry. 2013;48:1065-1079.

Cristea D, Vilarem G. Improving light fastness of natural dyes on cotton yarn. Dyes and pigments. 2006;70:238-245.

Nakashima T, Kurachi M, Kato Y, Yamaguchi K, Oda T. Characterization of bacterium isolated from the sediment at coastal area of omurabay in Japan and several biological activities of pigment produced by this isolate. Microbiol Immunol. 2005;49: 407-415.

Dufossé L. Microbial production of food grade pigments. Food Technology and Biotechnology. 2006;44:313-323.

Boonyapranai K, Tungpradit R, Lhieochaiphant S, Phutraku S. Optimization of submerged culture for the production of naphthoquinones pigment by Fusarium verticillioides. Chiang Mai Journal of Science. 2008;35:457-466.

Chintapenta LK, Rath CC, Maringinti B, Ozbay G. Culture conditions for growth and pigment production of a Mangrove Penicillium species. J Multidiscip Sci Res. 2014;2:1-5.

Yoon JH, Baek SJ. Molecular targets of dietary polyphenols with anti-inflammatory properties. Yonsei Med J. 2005;46:585-596.

Winrow VR, Winyard PG, Morris CJ, Blake DR. Free radicals in inflammation: second messengers and mediators of tissue destruction. British Medical Bulletin. 1993; 49:506-522.

Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clinical chemistry. 1995;41:1819-1828.

Menichini F, Tundis R, Bonesi M, Loizzo MR, Conforti F, Statti G, et al. The influence of fruit ripening on the phytochemical content and biological activity of Capsicum chinense Jacq. cv Habanero. Food Chem. 2009;114:553-560.

Mueller M, Hobiger S, Jungbauer A. Anti-inflammatory activity of extracts from fruits, herbs and spices. Food Chem. 2010;122:987-996.

Grammer A. Antibiotic sensitivity and assay test. Microbiological methods. 1976:235.

Xu Z, Zhou J, Cai J, Zhu Z, Sun X, Jiang C. Anti-inflammation effects of hydrogen saline in LPS activated macrophages and carrageenan induced paw oedema. J Inflamm. 2012;9:2.

Jayanth K, Jeyasekaran G, Shakila RJ. Isolation of marine bacteria, antagonistic to human pathogens. Indian J Marine Sci. 2002;31:39-44.

Selvameenal L, Radhakrishnan M, Balagurunathan R. Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening. Indian J Pharm Sci. 2009;71:499-504.

Dionisi HM, Lozada M, Olivera NL. Bioprospection of marine microorganisms: biotechnological applications and methods. Rev Argent Microbiol. 2012;44:49-60.

Cang S, Sanada M, Johdo O, Ohta S, Yoshimoto A. High production of prodigiosin by Serratia marcescensgrowth in ethanol. Biotechnol Lett. 2000;22:1716-1765.

Lemos ML, Toranzo AE, Barja JL. Antibiotic activity of epiphytic bacteria isolated from intertidal seaweeds. Microbial Ecology. 1985;11:149-163.

Blunt JW, Prinsep MR. Marine natural products. Nat Prod Rep. 2006;23:26-78.

Godinho A, Bhosle S. Carotenes produced by alkaliphilic orange-pigmented strain of Microbacterium arborescens--AGSB isolated from coastal sand dunes. Indian J Mar Sci. 2008;37:307.

Rosenfeld WD, ZoBell CE. Antibiotic production by marine microorganisms. J Bacteriol. 1947;54:393.

Arulmozhi DK, Veeranjaneyulu A, Bodhankar SL, Arora SK. Pharmacological studies of the aqueous extract of Sapindus trifoliatus on central nervous system: possible antimigraine mechanisms. J Ethnopharmacol. 2005;97:491-496.

Huang SS, Huang GJ, Ho YL, Lin YH, Hung HJ, Chang TN, et al.. Antioxidant and antiproliferative activities of the four Hydrocotyle species from Taiwan. Botanical Studies. 2008;49:311-322.

Schramm A, Matusik P, Osmenda G, Guzik TJ. Targeting NADPH oxidases in vascular pharmacology. Vascul Pharmacol. 2012;30:216-231.

Paola Rosanna D, Salvatore C. Reactive oxygen species, inflammation, and lung diseases. Curr Pharm Des. 2012;18:3889-3900.

Kim YJ, Kim EH, Hahm KB. Oxidative stress in inflammation‐based gastrointestinal tract diseases: Challenges and opportunities. J Gastroenterol Hepatol. . 2012;27:1004-1010.

Hulsmans M, Van Dooren E, Holvoet P. Mitochondrial reactive oxygen species and risk of atherosclerosis. Current atherosclerosis reports. 2012;14:264-276.

Panchaxari DM, Pampana S, Pal T, Devabhaktuni B, Aravapalli AK. Design and characterization of diclofenac diethylamine transdermal patch using silicone and acrylic adhesives combination. DARU.. 2013;7:1.

Ramesh S, Mathivanan N. Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes. World J. Microbiol Biotechnol. 2103;25:2103-2111.


Refbacks

  • There are currently no refbacks.