Development, evaluation and optimization of superparamagnetite nanoparticles prepared by co-precipitation method

Hashem Montaseri, Shohreh Alipour, Molood Alsadat Vakilinezhad

Abstract


Magnetic nanoparticles (MNPs) are of high interest due to their application in medical fields, in particular for theranostics. Specific properties required for such particles include high magnetization, appropriate size and stability. Biocompatible magnetically soft magnetite particles (Fe3O4) have been investigated for biological purposes. The intrinsic instability of these nanoparticles and their susceptibility to the oxidization in air, are limitations for their applications. Various methods have been described for synthesis of these nanoparticles among which co-precipitation method is widely experimented. In order to illustrate the synthesis of MNPs elaborately, the effect of different factors on particle formation were studied. The particles morphology, stability, paramagnetic effect, chemical structure and cytotoxicity were evaluated. Particles of 58 and 60 nm obtained by oleic acid coated (OMNPs) and citric acid coated (CMNPs) magnetite nanoparticles respectively. Transmission electron microscopy images exhibited the real sizes are 15 and 13 nm. Magnetic saturations of these nanoparticles were 72 and 68 emu/g which is suitable for medical applications. Both OMNPs and CMNPs were non-toxic to the SK-Br-3 and MCF-7 cells in the concentrations of <2.5 µg/mL. Since these particles exhibit relatively high magnetic saturation, low dose of such material would be required; therefore, these NPs seem to be suitable for theranostics.


Keywords


Magnetic nanoparticles; Oleic acid; Citric acid; SK-Br-3; MCF-7

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References


Lei Z, Li Y, Wei X. A facile two-step modifying process for preparation of poly(SStNa)-grafted Fe3O4/SiO2 particles. J Solid State Chem. 2008;181(3):480–486.

Yildiz I, Yildiz BS. Applications of thermoresponsive magnetic nanoparticles. J Nanomater. 2015. Article ID 350596.

Pankhurst QA, Connolly J, Jones SK, Dobson J. Applications of magnetic nanoparticles in biomedicine. J Phys D: Appl Phys. 2003;36:R167–R181.

Cullity BD, Graham CD. Introduction to magnetic materials. 2nd ed: John Wiley&sons Inc. 2009. p. 87-114.

Lu AH, Salabas EL, Schüth F. Magnetic nanoparticles:synthesis, protection, functionalization, and application. Angew Chem Int Ed Engl. 2007;46(8):1222–1244.

Bogren S, Fornara A, Ludwig F, Morales MdP, Steinhoff U, Hansen MF, et al. Classification of magnetic nanoparticle systems—synthesis, standardization and analysis methods in the nanomag project. Int J Mol Sci. 2015;16(9):20308-20325.

McBain SC, Yiu HH, Dobson J. Magnetic nanoparticles for gene and drug delivery. Int J Nanomed. 2008;3(2):169-180.

Plank C, Schillinger U, Scherer F, Bergemann C, Rémy J-S, Krötz F, et al. The magnetofection method: using magnetic force to enhance gene delivery. Biol Chem. 2003;384(5):737-747.

Baryshev M, Vainauska D, Kozireva S, Karpovs A. New device for enhancement of liposomal magnetofection efficiency of cancer cells. World Acad Sci Eng Technol. 2011;58:249-252.

Mohammadi-Samani S, Miri R, Salmanpour M, Khalighian N, Sotoudeh S, Erfani N. Preparation and assessment of chitosan-coated superparamagnetic Fe3O4 nanoparticles for controlled delivery of methotrexate. Res Pharm Sci. 2013;8(1):25-33.

Alipour S, Montaseri H, Tafaghodi M. Inhalable, large porous PLGA microparticles loaded with paclitaxel:preparation, in vitro and in vivo characterization. J Microencapsul. 2015;32(7):661-668.

Park KS, Tae J, Choi B, Kim YS, Moon C, Kim SH, et al. Characterization, in vitro cytotoxicity assessment, and in vivo visualization of multimodal, RITC-labeled, silica-coated magnetic nanoparticles for labeling human cord blood–derived mesenchymal stem cells. Nanomedicine. 2010;6(2):263-276.

Chung TH, Pan HC, Lee WC. Preparation and application of magnetic poly(styrene-glycidyl methacrylate) microspheres. J Magn Magn Mater. 2007;311:36–40.

Chen YH, Liu YY, Lin RH, Yen FS. Characterization of magnetic poly(methyl methacrylate) microspheres prepared by the modified suspension polymerization. J Appl Polym Sci. 2008;108:583-590.

Ramirez LP, Landfester K. Magnetic polystyrene nanoparticles with a high magnetite content obtained by miniemulsion processes. Macromol Chem Physic. 2003;204(1):22-31.

Hong RY, Feng B, Cai X, Liu G, Li HZ, Ding J, et al. Double-miniemulsion preparation of Fe3O4/poly(methyl methacrylate) magnetic latex. J Appl Polym Sci. 2009;112(1):89-98.

R. Ahmadi, Madaah Hosseini HR, Masoudi A. Avrami behavior of magnetite nanoparticles formation In co-precipitation process. J Min Metall Sect B-Metall. 2011;47(2)B:211-218.

Mascolo MC, Pei Y, Ring TA. Room temperature co-precipitation synthesis of magnetite nanoparticles in a large pH window with different bases. Materials. 2013;6(12):5549-5567.

Hamoudeh M, Faraj AA, Canet-Soulas E, Bessueille F, Léonard D, Fessi H. Elaboration of PLLA-based superparamagnetic nanoparticles:characterization, magnetic behaviour study and in vitro relaxivity evaluation. Int J Pharm. 2007;338(1-2):248-257.

Pierre JL, Gautier-Luneau I. Iron and citric acid: A fuzzy chemistry of ubiquitous biological relevance. BioMetals. 2000;13(1):91-96.


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