Cognitive enhancing of pineapple extract and juice in scopolamine-induced amnesia in mice

Amir Abbas Momtazi-borojeni, Hojjat Sadeghi-Aliabadi, Mohammed Rabbani, Alireza Ghannadi, Elham Abdollahi

Abstract


The objective of the present study was to evaluate the cognitive enhancing of pineapple juice and ethanolic extract in scopolamine-induced cognitive deficit mice. The ethanolic extract of pineapple (Ananas comosus (L.) Merr.) was prepared by maceration method and its juice was obtained by a homogenizer. Object recognition task was used to evaluate the mice memory. Exploration time in the first and second trial was recorded. The differences in exploration time between a familiar and a novel object in the second trial were taken as a memory index. Animals were randomly assigned into 15 groups of 6 each including: control group (normal saline + vehicle), positive control group (scopolamine + rivastigmine), seven experimental groups (received scopolamine alone or scopolamine + ethanolic extract of pineapple in different doses), six other experimental groups were treated by ethanolic extract or juice of pineapple in different doses. Scopolamine (100 µL, 1 mg/kg, i.p.) and pineapple juice or extract (50, 75 and 100 mg/kg, i.p.) were administered 40 and 30 min before starting the second trial in the experimental groups. Object discrimination was impaired after scopolamine administration. Results showed that juice and ethanolic extract of pineapple significantly restored object recognition ability in mice treated with scopolamine. These finding suggested that pineapple had a protective role against scopolamine-induced amnesia, indicating its ability in management of cognitive disorders.

Keywords


Alzheimer's disease; Amnesia; Object recognition task; Pineapple; Scopolamine

Full Text:

PDF

References


Baker KF, Collins JL. Notes on the distribution and ecology of ananas and pseudananas in South America. Am J Bot. 1939;26:697-702.

d’Eeckenbrugge GC, Sanewski GM, Smith MK, Duval M-F, Leal F. Ananas. In: Kole C, editor. Wild crop relatives: genomic and breeding resources. New York: Springer; 2011. pp. 21-41.

Taussig SJ, Batkin S. Bromelain, the enzyme complex of pineapple (Ananas comosus) and its clinical application. An update. J Ethnopharmacol. 1988;22(2):191-203.

Fitzhugh DJ, Shan S, Dewhirst MW, Hale LP. Bromelain treatment decreases neutrophil migration to sites of inflammation. Clin Immunol. 2008;128(1):66-74.

Hale LP, Greer PK, Trinh CT, Gottfried MR. Treatment with oral bromelain decreases colonic inflammation in the IL-10-deficient murine model of inflammatory bowel disease. Clin Immunol. 2005;116(2):135-142.

Mynott TL, Ladhams A, Scarmato P, Engwerda CR. Bromelain, from pineapple stems, proteolytically blocks activation of extracellular regulated kinase-2 in T cells. J Immunol. 1999;163(5):2568-2575.

Cordenunsi B, Saura-Calixto F, Diaz-Rubio ME, Zuleta A, Tiné MA, Buckeridge MS, et al. Carbohydrate composition of ripe pineapple (cv. Perola) and the glycemic response in humans. Food Sci. Technol (Campinas). 2010;30:282-288.

Hemalatha R, Anbuselvi S. Physicohemical constituents of pineapple pulp and waste. J Chem Pharm Res. 2013;5(2):240-242.

Kader A, Hossain FMJ, Islam MM, Kabir G, Sarkar SK, Absar N. A comparative analysis on the nutritional contents of two varieties of pineapple of Chittagong region. Chittagong Univ J Biol Sci. 2013;5:105-112.

Wang L, Tang DQ, Kuang Y, Lin FJ, Su Y. Structural characteristics of pineapple pulp polysaccharides and their antitumor cell proliferation activities. J Sci Food Agric. 2015;5(12):2554-2561.

Sun J, Li L, You X, Li C, Zhang E, Li Z, et al. Phenolics and polysaccharides in major tropical fruits: chemical compositions, analytical methods and bioactivities. Analytical Methods. 2011;3:2212-20.

da Silva LMR, de Figueiredo EAT, Ricardo NMPS, Vieira IGP, de Figueiredo RW, Brasil IM, et al. Quantification of bioactive compounds in pulps and by-products of tropical fruits from Brazil. Food Chem. 2014;143:398-404.

Hossain MA, Rahman SM. Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Int Food Res J. 2011;44:672-676.

Debnath P, Dey P, Chanda A, Bhakta T. A Survey on pineapple and its medicinal value. Scholars Academic & Scientific Publishers. 2012;1:24-29.

Francis PT, Palmer AM, Snape M, Wilcock GK. The cholinergic hypothesis of Alzheimer’s disease: a review of progress. J Neurol Neurosurg Psychiatry. 1999;66:137-147.

Hasselmo ME, McGaughy J. High acetylcholine levels set circuit dynamics for attention and encoding and low acetylcholine levels set dynamics for consolidation. Prog Brain Res. 2004;145:207-231.

Bartus RT. On neurodegenerative diseases, models, and treatment strategies: lessons learned and lessons forgotten a generation following the cholinergic hypothesis. Exp Neurol. 2000;163(2):495-529.

Babic T. The cholinergic hypothesis of Alzheimer's disease: a review of progress. J Neurol Neurosurg Psychiatry. 1999;67:558.

Francis PT, Palmer AM, Snape M, Wilcock GK. The cholinergic hypothesis of Alzheimer's disease: a review of progress. J Neurol Neurosurg Psychiatry. 1999;66:137-147.

Corey-Bloom J, Anand R, Veach Jf. A randomized trial evaluating the efficacy and safety of ENA 713 (rivastigmine tartrate), a new acetylcholinesterase inhibitor, in patients with mild to moderately severe Alzheimer's disease. Int J Geriatr Psychiatry. 1998;1:55-65.

Petersen RC, Thomas RG, Grundman M, Bennett D, Doody R, Ferris S, et al. Vitamin E and donepezil for the treatment of mild cognitive impairment. N Engl J Med Overseas Ed. 2005;352:2379-2388.

Salloway S, Ferris S, Kluger A, Goldman R, Griesing T, Kumar D, et al. Efficacy of donepezil in mild cognitive impairment A randomized placebo-controlled trial. Neurology. 2004;63(4):651-657.

Camps P, Formosa X, Galdeano C, Gómez T, Munoz-Torrero D, Scarpellini M, et al. Novel donepezil-based inhibitors of acetyl-and butyrylcholinesterase and acetylcholinesterase-induced β-amyloid aggregation. J Med Chem. 2008;51:3588-3598.

Jacobson SA, Sabbagh MN. Donepezil: potential neuroprotective and disease-modifying effects. Expert Opin Drug Metab Toxicol. 2008;4(10):1363-1369.

Rogers SL, Doody RS, Mohs RC, Friedhoff LT. Donepezil improves cognition and global function in Alzheimer disease: a 15-week, double-blind, placebo-controlled study. Arch Intern Med. 1998;158(9):1021-1031.

Ballard CG, Gauthier S, Cummings JL, Brodaty H, Grossberg GT, Robert P, et al. Management of agitation and aggression associated with Alzheimer disease. Nat Rev Neurol 2009;5(5):245-255.

Lahiri DK, Farlow MR, Greig NH, Sambamurti K. Current drug targets for Alzheimer's disease treatment. Drug Dev Res. 2002;56:267-281.

Butler MS. Natural products to drugs: natural product-derived compounds in clinical trials. Nat Prod Rep. 2008;25:475-516.

Wang B-s, Wang H, Wei Z-h, Song Y-y, Zhang L, Chen H-z. Efficacy and safety of natural acetylcholinesterase inhibitor huperzine A in the treatment of Alzheimer’s disease: an updated meta-analysis. J Neural Transm. 2009;116(4):457-465.

Bai D. Development of huperzine A and B for treatment of Alzheimer's disease. Pure Appl Chem. 2007;79:469-479.

Shi Y-f, Zhang H-y, Wang W, Fu Y, Xia Y, Tang X-c, et al. Novel 16-substituted bifunctional derivatives of huperzine B: multifunctional cholinesterase inhibitors. Acta Pharmacol Sin. 2009;30:1195-1203.

Guo AJ, Xie HQ, Choi RC, Zheng KY, Bi CW, Xu SL, et al. Galangin, a flavonol derived from Rhizoma Alpiniae Officinarum, inhibits acetylcholinesterase activity in vitro. Chem Biol Interact. 2010;187(1-3):246-268.

Seidl C, Correia BL, Stinghen AE, Santos CA. Acetylcholinesterase inhibitory activity of uleine from Himatanthus lancifolius. Zeitschrift für Naturforschung C. 2010;65(7-8):440-444.

Herring A, Münster Y, Akkaya T, Moghaddam S, Deinsberger K, Meyer J, et al. Kallikrein-8 inhibition attenuates Alzheimer's pathology in mice. Alzheimers Dement. 2016, in press. DOI: http://dx.doi.org/10.1016/j.jalz.2016.05.006.

Ennaceur A, Delacour J. A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data. Behav Brain Res. 1988;31:47-59.

Bertaina-Anglade V, Enjuanes E, Morillon D, Drieu la Rochelle C. The object recognition task in rats and mice: A simple and rapid model in safety pharmacology to detect amnesic properties of a new chemical entity. J Pharmacol Toxicol Methods. 2006;54(2):99-105.

Korttila K, Levanen J, Auvinen J. Failure of intramuscularly administered lorazepam and scopolamine-morphine premedication to produce amnesic effects to supplement conduction anaesthesia. Acta Anaesthesiol Scand. 1980;24: 325-330.

Glick SD, Zimmerberg B. Amnesic effects of scopolamine. Behav Biol. 1972;7:245-254.

Bartus RT, Dean RL, Pontecorvo MJ, Flicker C. The cholinergic hypothesis: a historical overview, current perspective, and future directions. Ann N Y Acad Sci. 1985;444:332-358.

Howes M-JR, Houghton PJ. Plants used in Chinese and Indian traditional medicine for improvement of memory and cognitive function. Pharmacol Biochem Behav. 2003;75(3):513-527.

Van Meer P, Raber J. Mouse behavioural analysis in systems biology. Biochem J. 2005;389:593-610.

Okuda S, Roozendaal B, McGaugh JL. Glucocorticoid effects on object recognition memory require training-associated emotional arousal. Proc Natl Acad Sci U S A. 2004;101(3):853-858.

Orhan I, Kartal M, Tosun F, Şener B. Screening of various phenolic acids and flavonoid derivatives for their anticholinesterase potential. Zeitschrift für Naturforschung C. 2007;62(11-12):829-832.

Jung M, Park M. Acetylcholinesterase inhibition by flavonoids from Agrimonia pilosa. Molecules. 2007;12(9):2130-2139.

Moyo M, Ndhlala AR, Finnie JF, Van Staden J. Phenolic composition, antioxidant and acetylcholinesterase inhibitory activities of Sclerocarya birrea and Harpephyllum caffrum (Anacardiaceae) extracts. Food Chem. 2010;123:69-76.

Perry NS, Houghton PJ, Theobald A, Jenner P, Perry EK. In-vitro inhibition of human erythrocyte acetylcholinesterase by Salvia lavandulaefolia essential oil and constituent terpenes. J Pharm Pharmacol. 2000;52(12):895-902.

Jung HA, Jung YJ, Hyun SK, Min B-S, Kim D-W, Jung JH, et al. Selective cholinesterase inhibitory activities of a new monoterpene diglycoside and other constituents from Nelumbo nucifera stamens. Biol Pharm Bull. 2010;33(2):267-272.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons LicenseThis work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.