Biochemical and histopathological effects of green tea nanoparticles in ironized mouse model

Hidayat Sulistyo Sulistyo, Dhadhang Wahyu Kurniawan, Lantip Rujito

Abstract


Transfusion in the treatment of thalassemia gives rise to iron deposits in many organs. Since there are many obstacles in the use of deferoxamin (DFO) as an iron chelating agent, it is important to find another alternative therapy that can act as iron chelation. The study aims to compare the histopathological pictures of the heart and spleen in iron-induced rats after administration of DFO and nanoparticles of green tea extract. The research used experimental research design with a post-test only control group. Experimental nano green teas were divided into four treatment groups; no diet, DFO supplementation, nano green tea supplementation, and a combination of both DFO and green tea. Ferritin and glutathione peroxides were used as biochemical parameters, and histopathological pictures of the heart and spleen were recorded. The study showed that there was significant improvement in the rats receiving DFO and nanoparticles of green tea compared with the rats in the no diet group. The study also reported that nano green tea has an effect comparable to DFO.

Keywords


Green tea; Nanoparticle; Thalassemia; Iron chelator; Transfusion

Full Text:

PDF

References


Srichairatanakool S, Kulprachakarn K, Pangjit K, Pattanapanyasat K, Fuchaeron S. Green tea extract and epigallocatechin 3-gallate reduced labile iron pool and protected oxidative stress in iron-loaded cultured hepatocytes. Adv Biosci Biotechnol. 2012;3(8):1140-1150.

Rujito L, Mulatsih S, M. Sofro AM. Status of superoxide dismutase in transfusion dependent thalassaemia. N Am J Med Sci. 2015;7(5):194-198.

Prabhu R, Prabhu V, Prabhu RS. Iron overload in beta thalassemia - a review. J Biosci Tech. 2009;1(1):20-31.

Badria FA, Ibrahim AS, Badria AF, Elmarakby AA. Curcumin attenuates iron accumulation and oxidative stress in the liver and spleen of chronic iron-overloaded rats. PLoS One. 2015;10(7):e0134156.

Sawada T, Konomi A, Yokoi K. Iron deficiency without anemia is associated with anger and fatigue in young Japanese women. Biol Trace Elem Res. 2014;159(1-3):22-31.

Crespy V, Williamson G. A review of the health effect of green tea catechins in in vivo animal models. J Nutr. 2004;134(12 suppl):3431S-3440S.

Albrecht MA, Evans W, Raston CL. Green chemistry and the health implications of nanoparticles. Green Chem. 2006;8(5):417-432.

Suri SS, Fenniri H, Singh B. Nanotechnology-based drug delivery systems. J Occup Med Toxicol. 2007;2:16.

Namdeo M, Bajpai SK. Chitosan-coated magnetite (CCM) nanoparticles as novel iron-chelator for treatment of β-Thalassemia by tag and drag (TAD) approach. J Bionanosci. 2007;1(2):131-133.

Khan N, Bharali DJ, Adhami VM, Siddiqui IA, Cui H, Shabana SM, et al. Oral administration of naturally occurring chitosan-based nanoformulated green tea polyphenol EGCG effectively inhibits prostate cancer cell growth in a xenograft model. Carcinogenesis. 2014;35(2):415-423.

Ounjaijean S, Thephinlap C, Khansuwan U, Phisalapong C, Fucharoen S, Porter JB, et al. Effect of green tea on iron status and oxidative stress in iron-loaded rats. Med Chem. 2008;4(4):365-370.

Basu A, Betts NM, Mulugeta A, Tong C, Newman E, Lyons TJ. Green tea supplementation increases glutathione and plasma antioxidant capacity in adults with the metabolic syndrome. Nutr Res. 2013;33(3) :180–187.

Hatcher HC, Singh RN, Torti FM, Torti SV. Synthetic and natural iron chelators: therapeutic potential and clinical use. Future Med Chem. 2009;1(9):1643-1670.

Saewong T, Ounjaijean S, Mundee Y, Pattanapanyasat K, Fucharoen S, Porter JB, et al. Effects of green tea on iron accumulation and oxidative stress in livers of iron-challenged thalassemic mice. Med Chem. 2010;6(2):57-64.

Yi S, Wang Y, Huang Y, Xia L, Sun L, Lenaghan SC, et al. Tea nanoparticles for immunostimulation and chemo-drug delivery in cancer treatment. J Biomed Nanotechnol. 2014;10(6):1016-1029.

Mounsey RB, Teismann P. Chelators in the treatment of iron accumulation in parkinson's disease. Int J Cell Biol. 2012(1);ID 983245.

Al-Tubaikh JA. Internal Medicine: An Illustrated radiological Guide. 1st ed. Berlin: Springer-Verlag New York, LLC. 2010.

Aigner E, Theurl I, Theurl M, Lederer D, Haufe H, Dietze O, et al. Pathways underlying iron accumulation in human nonalcoholic fatty liver disease. Am J Clin Nutr. 2008;87(5):1374-1383.

Walker JM. Thalassaemia major and the heart: a toxic cardiomyopathy tamed? Heart. 2013;99:827-834.

Aaseth J, Skaug MA, Cao Y, Andersen O. Chelation in metal intoxication--Principles and paradigms. J Trace Elem Med Biol. 2015;31:260-266.

Berdoukas V, Farmaki K, Carson S, Wood J, Coates T. Treating thalassemia major-related iron overload: the role of deferiprone. J Blood Med. 2012;3:119-129.

Srichairatanakool S, Ounjaijean S, Thephinlap C, Khansuwan U, Phisalpong C, Fucharoen S. Iron-chelating and free-radical scavenging activities of microwave-processed green tea in iron overload. Hemoglobin. 2006;30(2):311-327.

Samman S, Sandström B, Toft MB, Bukhave K, Jensen M, Sørensen SS, et al. Green tea or rosemary extract added to foods reduces nonheme-iron absorption. Am J Clin Nutr. 2001;73(3):607-612.

Mandel S, Weinreb O, Amit T, Youdim MB. Cell signaling pathways in the neuroprotective actions of the green tea polyphenol (-)-epigallocatechin-3-gallate: implications for neurodegenerative diseases. J Neurochem. 2004;88(6):1555-1569.

Pinyou P, Kradtap Hartwell S, Jakmunee J, Lapanantnoppakhun S, Grudpan K. Flow injection determination of iron ions with green tea extracts as a natural chromogenic reagent. Anal Sci. 2010;26(5):619-623.

Perron NR, Brumaghim JL. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem Biophys. 2009;53(2):75-100.

Zaveri NT. Green tea and its polyphenolic catechins: medicinal uses in cancer and noncancer applications. Life Sci. 2006;78(18):2073-2080.

Liu G, Men P, Harris PL, Rolston RK, Perry G, Smith MA. Nanoparticle iron chelators: a new therapeutic approach in alzheimer disease and other neurologic disorders associated with trace metal imbalance. Neurosci Lett. 2006;406(3):189-193.

Ahosseini M, Abbasian S, Moloudian H, Karimi F, Khoshayand M. Evaluation of antioxidant properties of Iran's native plants and investigation their synergistic effects with tea. Res Pharm Sci. 2012;7(5):S10.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons LicenseThis work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.