Radioprotective effects of lentil sprouts against X-ray radiation

Abbas Haghparast, Kamran Mansouri, Samane Moradi, Fatemeh Dadashi, Saeed Eliasi, Mahdi Sobhani, Kambiz Varmira


The present study investigated the radioprotective efficacy of lentil (Lens culinaris) sprouts against X-ray radiation-induced cellular damage. Lentil seeds were dark germinated at low temperature and the sprout extract was prepared in PBS. Free radical scavenging of extract was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and then the radioprotective potency of extract (0 to 1000 µg/mL) on the lymphocyte cells was determined by lactate dehydrogenases assay. Moreover, micronuclei assay was assessed using the cytokinesis-block technique. The irradiations were performed using 6 MV X-ray beam. The value of IC50 for DPPH assay was 250 µg/mL. The median lethal dose for radiation was determinate at 5.37 Gy. Pretreatment with lentil sprout extract at 1000 µg/mL reduced cytotoxicity at 6 Gy total concentration from 70% to 50%. The results of micronuclei assay indicated that cells were resistant to radiation at concentrations of 500-1000 µg/mL of exogenous lentil sprout extract. The value of median effective concentration for micronuclei assay was 500 µg/mL. The results indicated that lentil sprout extract showed actually somewhat radioprotective effect on lymphocyte cell. In addition, the obtained results suggest that extract of total lentil sprout have more antioxidant activity than radicle part.


Radioprotective agents; Germination; X-Radiation; Legumes

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Niva M, Mäkelä J. Finns and functional foods: socio‐demographics, health efforts, notions of technology and the acceptability of health‐promoting foods. Int J Consum Stud. 2007;31:34-45.

Goldberg I. Functional foods: designer foods, pharmafoods, nutraceuticals. Springer Science & Business Media. 2012; 17-19.

Kuo YH, Rozan P, Lambein F, Frias J, Vidal-Valverde C. Effects of different germination conditions on the contents of free protein and non-protein amino acids of commercial legumes. Food Chem. 2004;86:537-545.

Shapiro TA, Fahey JW, Dinkova-Kostova AT, Holtzclaw WD, Stephenson KK, Wade KL, et al. Safety, tolerance, and metabolism of broccoli sprout glucosinolates and isothiocyanates: a clinical phase I study. Nutrition and cancer. 2006;55:53-62.

Cevallos-Casals BA, Cisneros-Zevallos L. Impact of germination on phenolic content and antioxidant activity of 13 edible seed species. Food Chem. 2010;119:1485-1490.

Thavarajah P, Wejesuriya A, Rutzke M, Glahn RP, Combs GF, Vandenberg A. The potential of lentil (Lens culinaris L.) as a whole food for increased selenium, iron, and zinc intake: preliminary results from a 3 year study. Euphytica. 2011;180:123-128.

Ayet G, Burbano C, Cuadrado C, Pedrosa M, Robredo L, Muzquiz M, et al. Effect of germination, under different environmental conditions, on saponins, phytic acid and tannins in lentils (Lens culinaris). J Science Food Agric. 1997;74:273-279.

Bartolomé B, Estrella I, Hernandez T. Changes in phenolic compounds in lentils (Lens culinaris) during germination and fermentation. Z Lebensm Unters Forsch. 1997;205:290-294.

El-Mahdy AR, Moharram YG, Abou-Samaha OR. Influence of germination on the nutritional quality of lentil seeds. Z Lebensm Unters Forsch. 1985;181:318-320.

Baenas N, García-Viguera C, Moreno DA. Elicitation: a tool for enriching the bioactive composition of foods. Molecules. 2014;19:13541-13563.

Świeca M, Gawlik-Dziki U, Kowalczyk D, Złotek U. Impact of germination time and type of illumination on the antioxidant compounds and antioxidant capacity of Lens culinaris sprouts. Sci Hortic. 2012;140:87-95.

Świeca M, Baraniak B. Nutritional and antioxidant potential of lentil sprouts affected by elicitation with temperature stress. J Agric Food Chem. 2014;62:3306-3313.

Świeca M, Surdyka M, Gawlik‐Dziki U, Złotek U, Baraniak B. Antioxidant potential of fresh and stored lentil sprouts affected by elicitation with temperature stresses. Int J Food Sci Technol. 2014;49:1811-1817.

Xu B, Chang S. A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J food science. 2007;72(2):159-166.

Varanda EA, Tavares DC. Radioprotection: mechanisms and radioprotective agents including honeybee venom. J Venom Anim Toxins. 1998;4(1):5-21.

Dehghani H, Sabaghpour S, Sabaghnia N. Genotype x environment interaction for grain yield of some lentil genotypes and relationship among univariate stability statistics. Span J Agric Res. 2008;6(3): 385-394.

Hosseinimehr SJ, Mahmoudzadeh A, Ahmadi A, Ashrafi SA, Shafaghati N, Hedayati N. The radioprotective effect of Zataria multiflora against genotoxicity induced by γ irradiation in human blood lymphocytes. Cancer Biother Radiopharm. 2011;26:325-359.

Farhadi L, Mohammadi-Motlagh HR, Seyfi P, Mostafaie A. Low concentrations of flavonoid-rich fraction of shallot extract induce delayed-type hypersensitivity and TH1 cytokine IFNγ expression in Balb/c Mice. Int J Mol Cell Med Winter. 2014;3(1):16-25.

Mohammadi-Motlagh HR, Mostafaie A, Mansouri K. Anticancer and anti-inflammatory activities of shallot (Allium ascalonicum) extract. Arch Med Sci. 2011;7(1):38–44.

Pan Y, Ye S, Yuan D, Zhang J, Bai Y, Shao C. Radioprotective role of H2S/CSE pathway in Chang liver cells. Mutat Res. 2012;738:12-18.

Radiation, U.N.S.C.o.t.E.o.A. Sources and effects of ionizing radiation: sources. United Nations Publications.Vol. 1. 2008;11-61.

Hosseinimehr SJ. Trends in the development of radioprotective agents. Drug Discov Today. 2007;12(19-20):794-805.

Troszyńska A, Estrella I, Lamparski G, Hernández T, Amarowicz R, Pegg RB. Relationship between the sensory quality of lentil (Lens culinaris) sprouts and their phenolic constituents. Food Res Int. 2011;44:3195-3201.

Leprince O, Deltour R, Thorpe PC, Atherton NM, Hendry GA. The role of free radicals and radical processing systems in loss of desiccation tolerance in germinating maize (Zea mays L.). New Phytol. 1990;116:573-580.

Dionet C, Muller-Barthelemy M, Marceau G, Denis JM, Averbeck D, Gueulette J, et al.. Different dose rate-dependent responses of human melanoma cells and fibroblasts to low dose fast neutrons. Int J Radiat Biol. 2016; 3;92(9):527-535.

Slosarek K, Konopacka M, Rogolinski J, Sochanik A. Effect of dose-rate and irradiation geometry on the biological response of normal cells and cancer cells under radiotherapeutic conditions. Mutat Res Genet Toxicol Environ Mutagen. 2014;773: 14-22.

Nada AS, Hawas AM, Amin NE, Elnashar MM, Abd Elmageed ZY. Radioprotective effect of Curcuma longa extract on γ-irradiation-induced oxidative stress in rats. Can J Physiol Pharmacol. 2012;90:415-423.

Yu J, Piao BK, Pei YX, Qi X, Hua BJ. Protective effects of tetrahydropalmatine against γ-radiation induced damage to human endothelial cells. Life Sci. 2010;87(1-2):55-63.

Ježovičová M, Koňariková K, Ďuračková Z, Keresteš J, Králik G, Žitňanová I. Protective effects of black tea extract against oxidative DNA damage in human lymphocytes. Mol Med Rep. 2016; 13(2):1839-1844.

Gumus ZP, Guler E, Demir B, Barlas FB, Yavuz M, Colpankan D, et al. Herbal infusions of black seed and wheat germ oil: Their chemical profiles, in vitro bio-investigations and effective formulations as phyto-nanoemulsions. Colloids Surf B Biointerfaces. 2015;133:73-80.

Ghanekar SP, Korgaonkar K. Radioprotective effect of groundnut oil on skin. Indian J Cancer. 1972;9(3):216-222.


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