Spasmodic versus spasmolytic activities of Euphorbia spinidens extract on rat isolated uterus

Mustafa Ghanadian, Hassan Sadraei, Zeinab Cheraghi

Abstract


Preterm contraction of uterus is a main cause of miscarriages and preterm labour. Euphorbia known as Ferphion in Iranian traditional medicine texts like Al-Hawi, is reported for prevention of preterm labour. Therefore, the objective of this research was to investigate the effect of Euphorbia spinidens Bornm. Ex Prokh. on motility of rat uterus. Uterine horns were isolated form non-pregnant female rats pretreated with estrogen. E. spinidens hydroalcoholic extract was examined on KCl (80 mM) induced and spontaneous periodic contraction in isolate uterine strips suspended in an organ bath and compared with nifedipine and ritodrine. In isolated rat uterine strips, E. spinidens extract (1-500 µg/mL) showed mixed effects. At lower concentrations, firstly potentiated the spontaneous periodic contraction, while in concentrations above 256 µg/mL the spontaneous periodic contractions were completely attenuated. These findings demonstrated that although lower concentrations of hydroalcoholic extract potentiated the spontaneous periodic contraction of rat uterine smooth muscle, but at higher concentrations it had inhibitory effect on rat uterus contraction.


Keywords


Euphorbia spinidens; Uterus; Spasmodic; Spasmolytic; KCl

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References


Beck S, Wojdyla D, Say L, Betran AP, Merialdi M, Requejo JH, et al. The worldwide incidence of preterm birth: a systematic review of maternal mortality and morbidity. Bull. World Health Organ. 2010;88:31-38.

Romero R, Dey SK, Fisher SJ. Preterm labour: one syndrome, many causes. Science. 2014;345(6198):760-765.

Tan TC, Devendra K, Tan LK, Tan HK. Tocolytic treatment for the management of preterm labour: a systematic review. Singapore Med J. 2006;47(5):361-366.

Al-Omari WR, Al-Shammaa HB, Al-Tikriti EM, Ahmed KW. Atosiban and nifedipine in acute tocolysis: a comparative study. Eur J Obstet Gynecol Reprod Biol. 2006;128(1-2):129-134.

King J, Flenady V, Cole S, Thornton S. Cyclo-oxygenase (COX) inhibitors for treating preterm labour. Cochrane Database Syst Rev. 2005;2:70-80.

Romero R, Sibai BM, Sanchez-Ramos L, Valenzuela GJ, Veille JC, Tabor B, et al. An oxytocin receptor antagonist (atosiban) in the treatment of preterm labour: a randomized, double-blind, placebo-controlled trial with tocolytic rescue. Am J Obstet Gynecol. 2000;182(5):1173-1183.

Razi MZ, Afsharipour S (translator). Al-Hawi Fi Tebb. Vol 21. Tehran: The Academy of Medical Sciences of Iran. 2005, pp. 143-144.

Mirheydar H. Maaref giahi. Vol 4. 1st ed. Tehran: Office of Publication of Islamic Culture ; 1994. p. 285-293.

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. 8th Ed. Washington DC, The National Academies Press. 2011: p.11-37.

Muller A, Siemer J, Renner S, Hoffmann I, Beckmann MW, Dittrich R. Modulation of uterine contractility and peristalsis by oxytocin in estrogen-primed non-pregnant swine uteri. ‎Eur J Med Res. 2006;11(4):157-162.

Young RC. Myocytes, myometrium, and uterine contractions. Ann N Y Acad Sci. 2007;1101:72-84.

Parker KL, Schimmer BP. Pituitary hormones and their hypothalamic releasing hormones. In: Brunton LL, Lazo JS. Parker KL (edithors). Goodman and Gilman's The Pharmacological Basis of Therapeutics. 11th ed. New York, McGraw-Hill; 2006. Pp, 1489-1510.

Berridge MJ. Smooth muscle cell calcium activation mechanisms. J Physiol. 2008;586(21):5047-5061.

Ratz PH, Berg KM, Urban NH, Miner AS. Regulation of smooth muscle calcium sensitivity: KCl as calcium-sensitizing stimulus. Am J Physiol Cell Physiol. 2005;288(4):C769-783.

Webb RC. Smooth muscle contraction and relaxation. Adv Physiol Educ. 2003;27(1-4):201-206.

Houdeau E, Rossano B, Prud’homme MJ. Regional and muscle layer variations in cholinergic nerve control of32 the rat myometrium during the oestrous cycle. Auton Neurosci. 2003;104(1):1-9.

Lanzotti V. Diterpenes for therapeutic use. In: Ramawat KG, Merillon JM (editors). Natural Products. Springer Berlin-Heidelberg, 2013, pp. 3173-3191.

Jassbi AR. Chemistry and biological activity of secondary metabolites in Euphorbia from Iran. Phytochemistry. 2006;67(18):1977-1984.

Ghanadian M, Sadraei H, Yousuf S, Asghari G, Choudhary MI, Jahed M. New diterpene polyester and phenolic compounds from Pycnocycla spinosa Decne. Ex Boiss with relaxant effects on KCl-induced contraction in rat ileum. Phytochem Lett. 2014;7:57-61.

Shadi S, Saeidi H, Ghanadian M, Rahimnejad MR, Aghaei M, Ayatollahi SM, et al. New macrocyclic diterpenes from Euphorbia connata Boiss. with cytotoxic activities on human breast cancer cell lines. Nat prod res. 2015;29(7):607-614.


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