Antioxidant effects of hydroalcoholic and polyphenolic extracts of Peucedanum pastinacifolium Boiss. & Hausskn.

Ahmad Movahedian, Behzad Zolfaghari, Mehrzad Mirshekari


Antioxidant activity of Peucedanum pastinacifolium Boiss. & Hausskn aerial part hydroalcoholic extract (HAE) and polyphenolic extract (PPE) as well as their total phenolic and flavonoid contents were studied. Phenolic and flavonoid contents were respectively estimated as gallic acid and quercetin equivalents. The in vitro antioxidant activity of two extracts of P. pastinacifolium were evaluated by radical scavenging of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), chelating activity on ferrous ions, or ferric reducing antioxidant power (FRAP) assay. In addition, the in vivo antioxidant activity of hydroalcoholic extract was measured by FRAP assay. Total phenolic contents of PPE and HAE were 117.1 ± 6.2 and 44.3 ± 1.7 mg/g, respectively. Total flavonoid content of PPE (43.4 ± 2.1 mg/g) was found to be higher than that of HAE (8.0 ± 1.5 mg/g). In DPPH radical scavenging assay, HAE and PPE showed fifty percent inhibitory concentration (IC50) values of 469.4 ± 9.3 µg/mL and 128.2 ± 5.5 µg/mL, respectively. Iron chelating activity assays indicated IC50 values of 657.5 ± 13.2 µg/mL and 735.4 ± 16.1 µg/mL for HAE and PPE as opposed to ethylenediamine tetra-acetic acid (EDTA) being 16.5 ± 0.8 µg/mL. PPE exhibited greater FRAP value (154.0 ± 1.8 µM) as compared with that of HAE being 69.3 ± 1.4 µM. In animal study, HAE showed a significant (p < 0.05) increase in FRAP level when compared with that of control group. Our results showed that P. pastinacifolium possess antioxidant properties which most likely are exerted through free radical scavenging, chelating activity, and reducing power.


Peucedanum pastinacifolium; Antioxidant; Reducing power; Free radicals; Chelating activity

Full Text:



Pala SF, Gürkan H. The role of free radicals in ethiopathogenesis of diseases. Adv Mol Biol. 2009;2:1-9.

Young IS., Woodside JV. Antioxidants in health and disease. J Clin Pathol. 2001;54:176-186.

El-Bahr, Sabry M. Biochemistry of free radicals and oxidative stress. Sci Int. 2001;1:111-117.

Pokomy J. Are natural antioxidant better – and safer – than synthetic antioxidants? Eur J Lipid Sci Technol. 2007;109:629-642.

Gupta RK, Patel AK, Shah N, Chaudhary AK, Jha UK, Yadav UC, et al. Oxidative stress and antioxidants in disease and cancer. Asian Pac Cancer Prev. 2014;15:4405-4409.

Gholamzadeh S, Behbahani M, Sajjadi S, Shokoohinia Y, Hemmati S. Antivirial activity of elemicin from Peucedanum pastinacifolium. Res Pharm Sci. 2012;7:784.

Hisamato M, Kikuzaki H, Ohighashi H, Nakatani N. Antioxidant Compounds from the Leaves of Peucedanum japonicum thunb. Food Chem. 2003;51:5255–5261.

Roy L. G, Urooj A. Antioxidant potency, pH and heat stability of selected plant extracts. J Food Biochem. 2013;37:336-342.

Sevastre B, Lulia F, Marcus I, Prodan I, Tamas M, Deliu C. Peucedanum Oreoselinum L. MOENCH effect in Ehrlich ascitic carcinoma. Veterinary Medicine. 2008;65:124-132.

Tepe B, Askin Akpulat H, Sokmen M. Evaluation of the chemical composition and antioxidant activity of the essential oils of Peucedanum longifolium (Waldst. & Kit.) and P. palimbioides (Boiss.). Rec Not Prod. 2001;5:108-116.

Movahedian A, Zolfaghari B, Sajjadi SE, Moknatjou R. Antihyperlipidemic effect of Peucedanum pastinacifolium extract in streptozotocin-induced diabetic rats. Clinics. 2010;65:76-84.

Movahedian A, Sajjadi SE, Ahmadi A. Lipid lowering effect of ethanolic extract of aerial parts of Peucedanum pastinacifolium in hypercholesterolemic rats. Iran J Pharm Res. 2009;8:301-306.

Sajjadi SE, Shokoohinia Y, Hemmati S. Isolation and identification of furanocoumarins and phenylpropanoid from the acetone extract and identification of volatile constituents from the essential oil of Peucedanum pastinacifolium. Chem Nat Compd. 2011;48:668-681.

Seddik K, Nadjet I, Abderrahmane B, Daoud H, Lekhmici A. Antioxidant and antibacterial activities of extracts from Artemisia herba alba Asso. leaves and some phenolic compounds. J Med Plants Res. 2010;4:1273-1280.

Slinkard K, Singleton VL. Total phenolic analysis: automation and comparison with manual methods. Am J Enol Vitic. 1977;28:49-55.

Sahu R, Saxena J. Screening of total phenolic and flavonoid content in conventional and non-conventional species of curcuma. J Pharmacogn Phytochem. 2013;2:176-179.

Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J. 2013;21:143-152.

Ebrahimzadeh MA, Pourmorad F, Bekhradnia AR. Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. Afr J Biotechnol. 2008;7:3188-3192.

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal Biochem. 1996;239:70-76.

Tomé AD, Ferreira PM, Freitas RM. Inhibitory action of antioxidants (ascorbic acid or α-tocopherol) on seizures and brain damage induced by pilocarpine in rats. Arq Neuropsiquiatr. 2010;68:355-361.

Coruh N, Celep AS, Ozgokce F. Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food Chem. 2007;100:1237-1242.

Amponsah IK, Fleischer TC, Dickson RA, Annan K, Thoss V. Evaluation of anti-inflammatory and antioxidant activity of Furanocoumarins and Sterolin from the stem bark of Ficus exasperata Vahl (Moraceae). J Sci Innov Res. 2013;2:880-887.

Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010;15:7313-7352.

Ebrahimzadeh MA, Nabavi SM, Nabavi S, Eslami B, Rahmani Z. Antioxidant and antihemolytic activities of the leaves of Kefe cumin (Laser trilobum L) umbelliferae. Trop J Pharm Res. 2010;9:441-449.

Wannes WA, Mhamdi B, Sriti J, Jemia MB, Ouchikh O, Hamdaoui G. Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf, stem and flower. Food Chem Toxicol. 2010;48:1362-1370.

Hajimehdipoor SE, Naghibi F, Moghadam MH, Fotoohi H. Ferric reducing property of three species of Ferula (Apiaceae) from Iran. Res Pham Sci. 2012;7:149-155.

Marčetić MD, Petrović SD, Milenković MT, Niketić MS. Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis.(Apiaceae). Cent J Eur Biol. 2014;9:149-155.


  • There are currently no refbacks.

Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.