Anti-anaphylactic effects of Trichilia monadelpha (Thonn.) J. J. De Wilde extracts on rodent models of anaphylaxis

Inemesit Okon Ben, Eric Woode, George Asumeng Koffuor, Emmanuel Akomanin Asiamah

Abstract


Effects of petroleum ether and ethanolic extracts of Trichilia monadelpha stem bark (PEE and EAE) on compound 48/80-induced systemic and passive anaphylaxis were determined. Survival rate, extravasation, degranulation of mast cells, and secretion of tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured after pre-treatment with extracts (10-100 mg/kg) and disodium chromoglycate (2.5-250 µg/kg) and induction of anaphylaxis in C57BL/6 mice or Sprague-Dawley rats with compound 48/80. Histopathological assessments were made from skin biopsies of rats. Data was analyzed by Kaplan-Meier Survival Log-Rank Analysis, or One-way ANOVA and Holm-Sidak’s post hoc test. PEE and EAE inhibited (P ≤ 0.0001) tremors in systemic anaphylaxis passive cutaneous anaphylactic reactions and extravasation, stabilized or prevented (P ≤ 0.001-0.0001) mast cell degranulation, and inhibited (P ≤ 0.001-0.0001) TNF-α and IL-6 secretion. Per the findings, PEE and EAE of T. monadelpha have exhibited substantial anti-anaphylactic and anti-inflammatory property (with PEE performing better) which substantiates its use traditionally in management of allergies and other inflammatory disorders.

P < 0.001, respectively). Our findings indicate while lead acetate impairs spatial memory in rat, administration of Vit C, Vit B12, ω-3, or their combination prior to the lead exposure inhibits the lead induced cognitive loss. There was no remarkable difference in this effect between the used supplements.

 


Keywords


Mast cells degranulation; Trichilia monadelpha; Interleukin-6; TNF-α; Anaphylaxis

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Brown JM, Wilson TM, Metcalfe DD. The mast cell and allergic diseases: role in pathogenesis and implications for therapy. Clin Exp Allergy. 2008;38(1):4–18.

Galli SJ, Tsai M, Piliponsky AM. The development of allergic inflammation. Nature. 2008;454(7203):445–454.

Kemp SF, Lockey RF. Anaphylaxis: a review of causes and mechanisms. J Allergy Clin Immunol. 2002;110(3):341–348.

Nauta AJ, Engels F, Knippels LM, Garssen J, Nijkamp FP, Redegeld FA. Mechanisms of allergy and asthma. Eur J Pharmacol. 2008;585(2-3): 354–360.

Kusano M, Tabuchi M, Fukushima A, Funayama K, Diaz C, Kobayashi M, et al. Metabolomics data reveal a crucial role of cytosolic glutamine synthetase 1;1 in coordinating metabolic balance in rice. Plant J. 2011;66(3):456-466.

Iriti M, Varoni EM, Vitalini S. Melatonin in traditional Mediterranean diets. J Pineal Res. 2010;49(2):101-105.

Nigrovic PA, Lee DM. Mast cells in inflammatory arthritis. Arthritis Res Ther. 2005;7(1):1–11.

Conti S, Costa B, Colleoni M, Parolaro D, Giagnoni G. Antiinflammatory action of endocannabinoid palmitoylethanolamide and the synthetic cannabinoid nabilone in a model of acute inflammation in the rat. Br J Pharmacol. 2002;135(1):181-187.

Ainooson GK, Owusu G, Woode E, Ansah C, Annan K. Trichilia monadelpha bark extracts inhibit carrageenan-induced foot-oedema in the 7-day old chick and the oedema associated with adjuvant-induced arthritis in rats. Afr J Tradit Complement Altern Med. 2011;9(1):8-16.

Ben IO, Woode E, Koffuor GA, Boakye-Gyasi E, Ehigiator BE. Effect of Trichilia monadelpha (Thonn.) J. J. De Wilde (Meliaceae) extracts on haematology, cytokines and oxidative stress biomarkers in rats adjuvant-induced arthritis. Pharmacologia. 2016;7(1):32-43.

Ben IO, Woode E, Abotsi WKM, Boakye-Gyasi E. Preliminary phytochemical screening and in vitro antioxidant properties of Trichilia monadelpha (Thonn.) J. J. de Wilde (Meliaceae). J Med Biomed Sci. 2013;2(2):6-15.

Goetzl EJ, Cheng PP, Hassner A, Adelman DC, Frick OL, Speedharan SP. Neuropeptides, mast cells and allergy: novel mechanisms and therapeutic possibilities. Clin Exp Allergy. 1990;20:3–7.

Kim SH, Choi CH, Kim SY, Eun JS, Shin TY. Anti-allergic effects of Artemisra twayomogi on mast cell mediated allergy model. Exp Biol Med (Maywood, NJ). 2005;230(1):82-88.

Choi YH, Chai OH, Han EH, Choi SY, Kim HT, Song CH. Lipoic acid suppresses compound 48/80-induced anaphylaxis-like reaction. Anat Cell Biol. 2010;43(4):317-324.

Martin Y, Avendaño C, Piedras MJ, Agnieszka K. Evaluation of Evans Blue extravasation as a measure of peripheral inflammation. Prot Ex. 2010.209.

Sheehan DC, Hrapchak BB. Theory and practice of Histotechnology. 2nd ed. Ohio: Battelle Press; 1980.

Fukugasako S, Ito S, Ikemoto Y. Effect of methyl p-hydroxybenzoate (methyl paraben) on Ca2+ concentration and histamine release in rat peritoneal mast cells. Br J Pharmacol. 2003;139(2):381–387.

Ohmori H, Egusa H, Ueura N, Matsumoto Y, Kanayama N, Hikida M. Selective augmenting effects of nitric oxide on antigen-specific IgE response in mice. Immunopharmacology. 2000;46(1):55–63.

Royer B, Varadaradjalou S, Saas P, Guillosson JJ, Kantelip JP, Arock M. Inhibition of IgE-induced activation of human mast cells by IL-10. Clin Exp Allergy. 2001;31(5):694-704.

Stassen M, Muller C, Arnold M, Hultner L, Klein-Hessing S, Neudorfl C, et al. IL-9 and IL-13 production by activated mast cells is strongly enhanced in the presence of lipopolysaccharide: NF-kappa B is decisively involved in the expression of IL-9. J Immunol. 2001;166(7):4391-4398.

Conti P, Kempuraj D, Di Gioacchino M, Boucher W, Letourneau R, Kandere K, et al. Interleukin-6 and mast cells. Allergy Asthma Proc. 2002;23(5):331-335.

Kunkel G, Baumer FE, Okuda M, van Cauwenberge P. Mode of action and indication for disodium cromoglycate (DSCG). Allergol Immunopathol (Madr). 1985;13(4):285-289.

Ganguly T, Sainis KB. Inhibition of cellular immune responses by Tylophora indica in experimental models. Phytomedicine.. 2001;8(5):348-355.

Hodek P, Trefil P, Stiborová M. Flavonoids-potent and versatile biologically active compounds interacting with cytochromes P450. Chem Biol Interact. 2002;139(1):1-21.

Staerk D, Lykkeberg AK, Christensen J, Budnik BA, Abe F, Jaroszewki JW. In vitro Cytotoxic activity of phenanthroindolizidine alkaloids from Cynanchum vincetoxicum and Tylophora tanake against drug-sensitive and multidrug-resistant cancer cells. J Nat Prod. 2002;65(9):1299-1302.

Stockigt J, Hammes B, Ruppert M. Construction and expression of a dual vector for chemo-enzymatic synthesis of plant indole alkaloids in Escherichia coli. Nat Prod Res. 2010;24(8):759-766


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