Comparison of antispasmodic effect of hydroalcoholic extract of Dracocephalum kotschyi Boiss. in rat uterus and ileum

Hassan Sadraei, Gholamreza Asghari, Mahla Alinejad

Abstract


Dracocephalum kotschyi Boiss. is a traditional medicine with antispasmodic activities. The objective of this research was to study antispasmodic activities of hydroalcoholic extract of D. kotschyion rat isolated uterus contractions for comparison with isolated ileum. Hydroalcoholic extract was obtained from aerial part of D. kotschyi using percolation method. A portion of rat ileum or uterus was suspended in Tyrode’s solution at 37 °C and gassed with O2. Effect of D. kotschyi extract was assessed on ileum or uterus contractions induced by KCl (80 mM), acetylcholine (ACh, 500 nM), electrical field stimulation (EFS) or oxytocin (0.0005 IU/mL). The extract of D. kotschyi concentration-dependently inhibited ileum responses to KCl (IC50 = 65 ± 18 mg/mL), ACh (IC50 = 102 ± 18 mg/mL) and EFS (IC50 = 117 ± 29 mg/mL). The extract of D. kotschyi also concentration-dependently inhibited uterus responses to KCl (IC50 = 453 ± 64mg/mL), ACh (IC50 = 58 ± 9 mg/mL), EFS (IC50 = 22 ± 3 mg/mL) as well as oxytocin (IC50 = 70 ± 11 mg/mL). From this experiment it was concluded that D. kotschyi extract possesses antispasmodic activities on both smooth muscle of ileum and uterus. In comparison, the extract was more effective inhibitor of ACh and EFS responses in rat uterus than on the ileum. On the other hand, the extract was a more potent inhibitor of KCl response on rat ileum. However, the extract was found to be a potent inhibitor of oxytocin-induced contraction of rat uterus. These results indicate that D. kotschyi extract may contain components that might be useful lead compounds for prevention of uterus spasm.


Keywords


Dracocephalum kotschyi; Extract; Antispasmodic; Ileum; Uterus

Full Text:

PDF

References


Rechinger KH. Flora Iranica. Graz: Akademische Druck-u Verlagsanstalt; 1982. p. 218-230.

Davis PH. Flora of Turkey. Edinburgh: University press; 1982. p. 293.

Zargari A. Medicinal plants. Tehran: Tehran University Publication; 1990. p. 82-83.

Ghahreman A. Flora of Iran. Tehran: Research institute of forests and rangelands publications;1983. p. 432.

Mozaffarian V. A Dictionary of Iranian plants names. Tehran: Farhang Moaser; 1998. p. 192-193.

Mirheydar H. Maaref giahi. Tehran: Daftare nasher farhange eslami.; 1995. p. 170-176.

Jalali A, Jamzad Z. Red data book of Iran. Tehran: Research institute of forests and rangelands; 1999. p. 215.

Golshani S, Karamkhani F, Monsef-Esfehani HR, Abdollahi M. Antinociceptive effects of the essential oil of Dracocephalum kotschyi inthe mouse writhing test. J Pharm Pharmaceut Sci. 2004;7:76-79.

Faham N, Javidnia K, Bahmani M, Amirghofran Z. Calycopterin, an immunoinhibitory compound from the extract of Dracocephalum kotschyi. Phytother Res. 2008;22:1154-1158.

Sajjadi SE, Atar AM, Yektaian A. Antihyperlipidemic effect of hydroalcoholic extract, and polyphenolic fraction from Dracocephalum kotschyi Boiss. Pharm Acta Helv. 1998;73:167-170.

Amirghofran Z, Azadbakht M, Karimi MH. Evaluation of the immunomodulatory effects of five herbal plants. J Ethnopharmacol. 2000;72:167-172.

Talari M, Seydi E, Salimi A, Mohsenifar Z, Kamalinejad M, Pourahmad J. Dracocephalum: novel anticancer plant acting on liver cancer cell mitochondria. BioMed Res Int. 2014;2014:ID 892170.

Moghaddam G, Ebrahimi SA, Rahbar-Roshandel N, Foroumadi A. Antiproliferative activity of flavonoids: influence of the sequential methoxylation state of the flavonoid structure. Phytother Res. 2012;26:1023-1028.

Jahaniani F, Ebrahimi SA, Rahbar-Roshandel N, Mahmoudian M. Xanthomicrol is the main cytotoxic component of Dracocephalum kotschyi and a potential anti-cancer agent. Phytochemistry. 2005;66:1581-1592.

Sadraei H, Asghari GH, Kasiri F. Comparison of antispasmodic effects of Dracocephalum kotschyi essential oil, limonene and a-terpineol. Res Pharm Sci. 2015;10:109-116.

Yaghmai MS, Taffazoli R. The essential oil of Dracocephalum kotschyi Boiss. Flavour Frag J. 1988;3:33-36.

Saeidnia S, Gohari AR, Hadjiakhoondi A, Shafiee A. Bioactive compounds of the volatile oil of Dracocephalum kotschyi. J Biosci. 2007;62:793-796.

Sadraei H, Asghari GH, Kasiri F.Antispasmodic effect of Dracocephalum kotschyi hydroalcoholic extract on rat ileum contraction. Res Pharm Sci. 2015;10:446-452.

Gohari AR, Saeidnia S, Matsuo K, Uchiyama N, Yagura T, Ito M, et al. Flavonoid constituents of Dracocephalum kotschyi growing in Iran and their trypanocidal activity. J Nat Med. 2003;57:250-252.

Fattahi M, Nazeri V, Torras-Claveria L, Sefidkon F, Cusido, RM, Zamani Z, et al. A new biotechnological source of rosmarinic acid and surface flavonoids: Hairy root cultures of Dracocephalum kotschyi Boiss. Ind Crop Prod. 2013;50:256-263.

Samuelsson G. Drugs of natural origin. Stockholm, Swedish Pharmaceutical Press; 1999. p. 48-49.

Committee for the update of the guide for the care and use of laboratory animals National Research Council. Guide for the Care and use of Laboratory animals. Washington DC: The National Academies Press; 2010. p. 11-37.

Ekblad E, Sundler F. Motor responses in rat ileum evoked by nitric oxide donors vs. field stimulation: Modulation by pituitary adenylate cyclase-activating peptide forskolin and guanylate cyclase inhibitors. J Phamacol Exp Ther. 1997;283:23-28.

Baldassano S,Wang GD,Mulè F,Wood JD.Glucagon-like peptide-1 modulates neurally evoked mucosal chloride secretion in guinea pig small intestine in vitro. Am J Physiol Gastrointest Liver Physiol. 2012;302:G352-G358.

Sadraei H, Asghari GH, Emami S. Effect of Rosa damascena Mill. flower extract on rat ileum. Res Pharm Sci. 2013;8:277-284.

Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants. New Delhi:CSIR; 1950. p. 81.‏

Amin G. Popular medicinal plants of Iran. Tehran:Ministry of Health Publications; 1991. p. 41.

Sadraei H, Asghari GH, Shahverdi F. Antidiarrhoeal assessment of hydroalcoholic and hexane extracts of Dracocephalum kotschyi Boiss. and apigenin in mice. Res Pharm Sci. 2016;in press.

Ratz PH, Berg KM, Urban NH, Miner AS. Regulation of smooth muscle calcium sensitivity: KCl as calcium-sensitizing stimulus. Am J Physiol Cell Physiol. 2005;288:769-783.

Elorriaga M, Anselmi E, Hernandez JM, Docon P, Ivorra D. The sources of Ca2+ for muscarinic receptor-induced contraction in the rat ileum. J Pharm Pharmacol. 1996;48:817-819.

Kurjak M, Sattler D, Schusdziarra V, Allescher HD. Characterization of prejunctional and postjunctional muscarinic receptors of the ascending reflex contraction in rat ileum. J Pharmacol Exp Ther. 1999;290:893-900.

Zenilman ME. Origin and control of gastrointestinal motility. Surg Clin North Am. 1993;73:1081-1099.

Goyal RK, Hirano I. The enteric nervous system. N Engl J Med. 1996;334:1106-1115 .

Parker KL, Schimmer BP. Pituitary hormones and their hypothalamic releasing hormones. In: Brunton LL, Lazo JS. Parker KL, editors. Goodman and Gilman's The Pharmacological Basis of Therapeutics. 11thed. New york: McGraw-Hill; 2006. p. 1489-1510.

Rang HP, Dale MM, Ritter JM, Moore P. Rang and Dale's Pharmacology. 6thed. London:Churchill Livingstone Elsevier; 2007. p. 176, 361, 456.


Refbacks

  • There are currently no refbacks.


Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.