Email this article Anti-proliferative activity of Fumaria vaillantii extracts on different cancer cell lines
Abstract
Plant-derived natural products are known to have cancer chemo-preventive and chemo-therapeutic properties. Plant extracts or their active constituents are used as folk medicine in traditional therapies by 80% of the world population. The aim of the present study was to determine the anti-proliferative potential of Fumaria vaillantii extracts on three different cancer cell lines including malignant melanoma SKMEL-3, human breast adenocarcinoma MCF-7 and human myelogenous leukemia K562 as well as human gingival fibroblast (HGF) as normal cell line. Anti-proliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flowcytometry and annexin methods. Total phenolics and flavonoids were determined by Folin-Ciocalteu and aluminum chloride methods. Chloroform fraction had the lowest IC50 value at 72 h (0.1 µg/ml) in MCF-7 cells. Flowcytometry and annexin-V analysis indicated that the chloroform fraction induced necrosis in MCF-7 cells. In addition, the colorimetric methods showed that the methanolic fraction possessed the highest amount of total phenolics (33.03 ± 0.75 mg/g of dry powder) and flavonoids (10.5 ± 2.0 mg/g of dry powder).The collective data demonstrated that F. vaillantii chloroform fraction may contain effective compounds with chemo-therapeutic potential act through an apoptotic independent pathway.
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Tachjian A, Maria V, Jahangir A. Use of herbal products and potential interactions in patients with cardiovascular diseases. J Am Coll Cardiol. 2010;55:515-525.
Balachandran P, Govindarajan P. Cancer an ayurvedic perspective. Pharmacol Res. 2005;51: 19-30.
Shaik D, Malika FA, Rafi SM, Naqui B. Studies of antibacterial activity of ethanolic extract from Nericum indicum and Hibiscus rosasinensis. J Islamic Acad Sci. 1994;7:167–168.
Nembo NE, Dimo T, Sylvain O, Bopda M, Hescheler J, Nguemo F. The proliferative and chronotropic effects of Brillantaisia nitens Lindau (Acanthaceae) extracts on pluripotent stem cells and their cardiomyocytes derivatives. J Ethnopharmacol. 2014;156:73-81.
Abu-Irmaileh BE, Afifi FU. Herbal medicine in Jordan with special emphasis on commonly used herbs. J Ethnopharmacol. 2003;3:193-197.
Heydari Nasrabadi M, Aboutalebi H, Naseri M. Effect of Fumaria parviflora alcoholic extract on male rat’s reproductive system. J Med Plants Res. 2012;6:2004-2010.
Ozalson M. A Comparison of Fumaria parviflora Lam and Momordica balsamina Linn. Hepatoprotection. Pak J Bio Sci. 2011;14: 1034-1035.
Tripathi M, Singh BK, Raisuddin S, Kakkar P. Abrogation of nimesulide induced oxidative stress and mitochondria mediated apoptosis by Fumaria parviflora Lam. Extract. J Ethnopharmacol. 2011;136:94-102.
Orhan IE, Sener B, Musharraf SG. Antioxidant and hepatoprotective activity apprasial of four selected Fumaria species and their total phenol and flavonoid quantities. Exp Toxicol Pathol. 2012;64:205-209.
Suau R, Cabezudo B, Rico R, Najera F, Lopez-Romero M. Direct determination of alkaloid contents in Fumaria species by GC – MS. Phytochem Analysis. 2002;13:363-367.
Tripathi M, Singh BK, Raisuddin S, Kakkar P. Involvement of mitochondria mediated pathway in hepathoprotection conferred by Fumaria parviflora Lam. extract against nimesulid induced apoptosis in vitro. Toxicol In Vitro. 2010;24:495-508.
Gilani AH, Janbaz KH, Akhtar MS. Selective protective effect of an extract from Fumaria parviflora on paracetomal induced hepatotoxicity. Gen Pharmacol. 1996;27:979-983.
Ebrahimzadeh F, Keshavarzi M, Sheidaii M, Ghadam P. Fruit and seed morphology of the Fumaria L. species (Papaveraceae) of Iran. Turk J Bot.2011;35:167-173.
Sheldon JW, Balick MJ, Laird SA. Medicinal plants: can utilization and conservation coexist? In: Advances in economic botany, The New York Botanical Garden. New York: Bronx;1997.
Gao Y, Su Y, Qu L, Xu S, Meng L, Cai SQ, et al. Mitochondrial apoptosis contributes to the anti-cancer effect of Smilax glabra Roxb. Toxicol Lett. 2011;207:112–120.
Mahasneh AM, El-Oqlah AA. Antimicrobial activity of extracts of herbal plants used in the traditional medicine of Jordan. J Ethnopharmacol. 1999;64:271-276.
Al-Hussaini R, Mahasneh AM. Microbial growth and quorum sensing antagonist activities of herbal plants extracts. Molecules. 2009;9:3425-3435.
Salimi M, Majd A, Sepahdar Z, Azadmanesh K, Irian S, Ardestaniyan MH, et al. Cytotoxicity effects of various Juglans regia (walnut) leaf extracts in human cancer cell lines. Pharm Biol. 2012;50: 1416-1422.
Salimi M, Ardestaniyan MH, Mostafapour Kandelous H, Saeidnia S, Gohari AR, Amanzadeh A, et al. Anti-proliferative and apoptotic activities of constituents of chloroform extract of Juglans regia leaves. Cell Prolif. 2014;47:172-179.
Mostafapour Kandelous H, Salimi M, Khuri V, Rastkari N, Amanzadeh A, Salimi M. Mitochondrial apoptosis induced by Chamaemelum nobile extract in breast cancer cells. Iran J Pharm Res. 2015;In press.
Wang YJN, Shi GL, Zhao LL, Liu SQ, Yu TQ, Clarke SR, et al. Acaricidal activity of Juglans regia leaf extracts on Tetranychusviennensis and Tetranychuscinnabarinus (Acari: Tetranychidae). J Econ Entomol. 2007;100:1298-1303.
Porter LJ, Hrstich LN, Chan BG. The conversion of procyanidin and prodelphinidins to cyaniding and delphindin. Phytochemistry. 1986;25:223-230.
Makkar HPS, Blummel M, Borowy NK, Becker K. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. J Sci Food Agr. 1993;61:161-165.
Gudarzia H, Salimi M, Irian S, Amanzadeh A, Mostafapour Kandelous H, Azadmanesh K, Salimi M. Ethanolic extract of Ferula gummosa is cytotoxic against cancer cells by inducing apoptosis and cell cycle arrest. Nat Prod Res. 2015;29:546-550.
Kim HJ, Park SY, Lee HM, Seo DI, Kim YM. Antiproliferative effect of the methanol extract from the roots of Petasites japonicus on Hep3B hepatocellular carcinoma cells in vitro and in vivo. Exp Ther Med. 2015;9:1791-1796.
Plackal Adimuriyil George B, Tynga IM, Abrahamse H. In Vitro antiproliferative effect of the acetone extract of Rubus fairholmianus Gard. Root on human colorectal cancer cells. Biomed Res Int. 2015;2015:ID 165037.
Srivastava S, Choudhary GP. Pharmacognostic and pharmacological study of Fumaria vaillantii Loisel: a review. J Pharmacogn Phytochem. 2014;3:194-197.
Ivanov IG, Vrancheva RZ, Marchev AS, Petkova NT, Aneva IY, Denev PP, et al. Antioxidant activities and phenolic compounds in Bulgarian Fumaria species. Int J Curr Microbiol. Appl Sci. 2014;3:296-306.
Shambaleed H, Muhammad I, Barkatullah, Imtiaz A. Comparison of three extracts of Fumaria indica for the evaluation of cytotoxic and phytotoxic activities. IntJ Biosci. 2012;2:112-119.
Gao JL, Shi JM, He K, Zhang QW, Li SP, Lee SM, et al. Yanhusuo extract inhibits metastasis of breast cancer cells by modulating mitogen-activated protein kinase signaling pathways. Oncol Rep. 2008;20:819-824.
Gao JL, Shi JM, Lee SM, Zhang QW, Wang YT. Angiogenic pathway inhibition of Corydalis yanhusuo and berberine in human umbilical vein endothelial cells. Oncol Res. 2009;17:519-526.
Shi J, Zhang X, Ma Z, Zhang M, Sun F. Characterization of aromatase binding agents from the dichloromethane extract of Corydalis yanhusuo using ultrafiltration and liquid chromatography tandem mass spectrometry. Molecules. 2010;15:3556-3566.
Kim YA, Kon CS, Yea SS, Seo Y. Constituents of Corydalis heterocarpa and their anti-proliferative effects on human cancer cells. Food Chem Toxicol. 2010;48:722-728.
Pieme CA, Santosh G, Tekwu E, Askun T, Aydeniz H, Ngogang J, et al. Fruits and barks extracts of Zanthozyllum heitzii a spice from Cameroon induce mitochondrial dependent apoptosis and Go/G1 phase arrest in human leukemia HL-60 cells. Biol Res. 2014;47:1-13.
Choi WY, Jin CY, Han MH, Kim GY, Kim ND, Lee WH, et al. Sanguinarine sensitizeshuman gastric adenocarcinoma AGS cells to TRAIL-mediated apoptosis via downregulation of AKT and activation of caspase-3. Anticancer Res. 2009;29:4457-4465.
Burgeiro A, Bento AC, Gajate C, Oliveira PJ, Molliendo F. Rapid human melanoma cell death induced by sanguinarine through oxidative stress. Eur J Pharmacol. 2013;705:109-118.
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