Email this article Effect of Holothuria leucospilota extracted saponin on maturation of mice oocyte and granulosa cells
Abstract
Sea cucumbers saponins are triterpenoid glycosides which exert beneficial biomedical effects. This study was performed to assess the effect of saponin extracted from sea cucumber Holothuria leucospilota (H. leucospilota) on maturation of mice oocytes and granulosa cells. The germinal vesicles oocytes were collected from 6–8 weeks old Naval Medical Research Institute (NMRI) mice ovaries, randomly divided into untreated and four experimental groups and cultured In vitro. Maturation medium was supplemented with 0, 1, 2, 4 and 8 μg/ml saponin for 12 days. The rates of maturation were recorded through morphological observation by measurement of follicle diameter during treatment. After 4 days, the effects of saponin on granulosa cells were investigated by reactive oxygen species (ROS) measurement, super oxide dismutase (SOD) activity, caspase assay and tumor necrosis factor-alpha (TNF-α) expression. The oocyte maturation rate was significantly higher in treated groups (1 µg/ml). The ROS and SOD assays demonstrated the antioxidant potential of saponin. The caspase assay exhibited that optimum concentrations of saponin (1, 2 µg/ml) reduced caspase activity in granulosa cells. Flow cytometry showed that optimum concentration of saponin promoted oocyte maturation via down regulation of TNF-α as follicular degenerative factor in nursing cells. These results proposed that maturation rate were obtained after the incorporation of 1 µg/ml sea cucumber saponin. Moreover, the extracted saponin at concentrations of 1, 2 µg/ml enhanced follicle growth which is accompanied by attenuating ROS formation, elevating SOD activity and reducing TNF-α expression in granulosa cells. But, further examinations are required to understand precise mechanisms of saponin action on oocyte and granulosa cells.
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Smitz JE, Thompson JG, Gilchrist RB. The promise of in vitro maturation in assisted reproduction and fertility preservation. Semin Reprod Med. 2011;29:24-37.
Boivin J, Bunting L, Collins J, Nygren K. International estimates of infertility prevalence and treatment-seeking: potential need and demand for infertility medical care. Hum Reprod. 2007;22:1506-1512.
Covington S, Burns L. Infertility counseling: A comprehensive handbook for clinicians. 2nd ed. Cambridge: Cambridge University Press; 2006. p. 24-28.
Chian RC, Uzelac PS, Nargund G. In vitro maturation of human immature oocytes for fertility preservation. Fertil Steril. 2013;99:1173-1181.
Shackelford TK, Besser A, Goetz AT. Personality, marital satisfaction, and probability of marital infidelity. Individ Differ Res 2008;6:13-25.
Jurema M, Nogueira D. In vitro maturation of human oocytes for assisted reproduction. Fertil Steril 2006;86:1277-1291.
Rajabi-Toustani R, Motamedi-Mojdehi R, Roostaei-Ali Mehr M, Motamedi-Mojdehi R. Effect of Papaver rhoeas L. extract on in vitro maturation of sheep oocytes. Small Ruminant Res. 2013;114:146-151.
Ghaffari Sh, Hatami H, Dehghan Gh. Saffron ethanolic extract attenuates oxidative stress, spatial learning, and memory impairments induced by local injection of ethidiumbromide. Res Pharm Sci. 2015;10:222-232.
Ali A, Benkhalifa M, Miron P. In vitro maturation of oocytes: biological aspects. Reprod Biomed Online. 2006;13:437-446.
Handali S, Moghimipour E, Kooshapour H, Rezaee S, Khalili S. In vitro cholestrol binding affinity of total saponin extracted from Glycyrrhiza glabra. Asian J Pharm Clin Res. 2014;7:170-173.
Gilchrist R, Thompson J. Oocyte maturation: emerging concepts and technologies to improve developmental potential in vitro. Theriogenology. 2007;67:6-15.
Butler MS. The role of natural product chemistry in drug discovery. J Nat Prod. 2004;67:2141-2153.
Hussain S, Fareed S, Ansari S, Sajid M. Marine natural products: A lead for Anti-cancer. Indian J Geo-Marine Sci. 2012;41:27-39.
Bordbar S, Anwar F, Saari N. High-value components and bioactives from sea cucumbers for functional foods--a review. Mar Drugs. 2011;9:1761-1805.
Soltani M, Baharara J. Antioxidant and antiprolifereative capacity of dichloromethane extract of Holoturia leucospilota sea cucumber. Int J Cell Mol Biotechnol. 2014;2014:ID ijcmb-00013.
Moghimipour E, Sadaghi-Nejad B, Handali S, Ameri A, Ramezani Z, Azemi Me. In Vitro Screening of anti-candida activity of saponins extracted from Glycyrrhiza glabra and Quillaja Saponaria. Asian J Pharm Clin Res. 2014;7: 160-162.
Inalegwu B, Sodipo O. Phytochemical screening and haemolytic activities of crude and purified saponins of aqueous and methanolic extracts of leaves of Tephrosia vogelii Hook. F. Asian J Plant Sci Res. 2013;3:7-11.
Tian F, Zhang X, Tong Y, Yi Y, Zhang S, Li L, et al. PE, a new sulfated saponin from sea cucumber, exhibits anti-angiogenic and anti-tumor activities in vitro and in vivo. Cancer Biol Ther. 2005;4:874-882.
Li YX, Himaya SW a, Kim SK. Triterpenoids of marine origin as anti-cancer agents. Molecules. 2013;18:78867909.
Hu X, Wang Y, Wang J, Xue Y, Li Z, Nagao K, et al. Dietary saponins of sea cucumber alleviate orotic acid-induced fatty liver in rats via PPARa and SREBP-1c signaling. Lipids Heal Dis. 2010;9:1-9.
FrancisG, Kerem Z, Makkar HPS, BeckerK. The biological action of saponins in animal systems: a review. Br J Nutr. 2002;88:587-605.
Soltani M, Parivar K, Baharara J, Kerachian MA, Asili j. Putative mechanism for apoptosis-inducing properties of crude saponin isolated from sea cucumber (Holothuria leucospilota) as an antioxidant compound. Iran J Basic Med Sci. 2015;18:180-187.
Kitagawa Y, Suzuki K, Yoneda A, Watanabe T. Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS), and DNA fragmentation in porcine embryos. Theriogenology. 2004;62:1186-1197.
Su X, Xu C, Li Y, Gao X, Y L, et al. Antitumor activity of polysaccharides and saponin extracted from sea cucumber. J Clin Cell Immunol. 2011;2: 1-5.
Barakat IA, Al-Himaidi AR. Effects of fenugreek seed extract on in vitro maturation and subsequent development of sheep oocytes. Pakistan J. Zool. 2013;45:1679-1686.
Abdo M, Hisheh S, Arfuso F, Dharmarajan A. The expression of tumor necrosis factor-alpha, its receptors and steroidogenic acute regulatory protein during corpus luteum regression. Reprod Biol Endocrinol. 2008;6:50-55.
Eimani H, Hassani F, Haeri Rouhani Sa, Nasr Esfahani MH, Dalman A, Rezazadeh Valojerdi M, et al. Effect of B-Mercaptoethanol With and without Bso (Dl-Buthionine Sulfoximine) On resumption of meiosis, in vitro maturation and Embryo development of immature mouse oocytes. yakhteh. 2006;7:234-241.
Eimani H, Hasani F, Haeri Rohani S, Nasr Esfahani M, Rezazadeh M, Dalman A, et al. Effect of Cysteamine on in vitro maturation, resumption of meiosis and embryo development of immature mouse Oocytes. Yakhteh. 2005;7:1-55.
Eimani H, Hassani F, Haeri Rohani S, Nasr Esfahani M, Rezazadeh M, Dalman A. Effect of glutathione on resumption of meiosis in vitro maturation and development of immature mouse oocytes. Lournal of Mazandaran University of Medical Sciences. 2005;15:1-10.
Lv L, Yue W, Liu W, Ren Y, Lee K-B, Smith GW. Effect of oocyte selection, estradiol and antioxidant treatment on in vitro maturation of oocytes collected from prepubertal Boer goats. Ital J Anim Sci. 2010;9:50-54.
Wang ZJ, Yu SD, Xu ZR. Effect of supplementation of green tea polyphenols on the developmental competence of bovine oocytes in vitro. Braz J Med Biol Res. 2007;40:1079-1085.
Golkar-Narenji A, Eimani H, Samadi F, Hasani S, Shahverdi AH, Eftekhari-Yazdi P, et al. Effect of Papaver rhoeas extract on in vitro maturation and developmental competence of immature mouse oocytes. Reprod Med Biol. 2010;9:211-215.
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