Cytotoxic evaluation of different fractions of Salvia chorassanica Bunge on MCF-7 and DU 145 cell lines

Alireza Golshan, Elaheh Amini, Seyed Ahmad Emami, Javad Asili, Zahra Jalali, Sarvenaz Sabouri-Rad, Naghmeh Sanjar-Mousavi, Zahra Tayarani-Najaran


Because of antimicrobial, antioxidant, and anticancer potential, Salvia chorassanica Bunge (Lamiaceae) has been considered as a popular herb in Iranian traditional medicine. Previous studies have shown remarkable cytotoxic properties of the methanol, n-hexane and dichloromethane extract of S. chorassanica on human cervical cancer cells. To seek the therapeutic potentials of S. chorassanica, this study was undertaken to evaluate the cytotoxic activities of various extracts of this plant on human breast MCF-7 and prostate cancer DU 145 cells. The DU 145 cells were exposed to different concentrations of plant extracts (1-200 µg/ml). Cytotoxic activities were examined using alamarBlue® assay and apoptosis was assessed by acridine orange/propodium iodide double staining and evaluation of DNA fragmentation by flow cytometry. Our findings indicated that n-hexane and dichloromethane extracts had more cytotoxic activities against DU 145 and MCF-7 cell lines compared with other extracts (P<0.05). The acridine orange/propodium iodide staining showed apoptogenic properties of n-hexane and dichloromethane extracts which was consequently confirmed by flow cytometric histogram that exhibited an increase in sub-G1 peak in treated cells as compared with untreated cancer cell lines. Taken together, these observations demonstrated cytotoxic effects of S. chorassanica extracts on MCF-7 and DU 145 cell lines which is most likely exerted via apoptosis cell death. Therefore, further investigations on S. chorassanica extracts as potential chemotherapeutic agents are warranted.

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Neergheen VS, Bahorun Th, Taylor EW, Jen LS, Aruoma OI. Targeting specific cell signaling transduction pathways by dietary and medicinal phytochemicals in cancer chemoprevention. Toxicology. 2010;278:229-41.

Saunders FR, Wallace HM. On the natural chemoprevention of cancer. Plant Physiol Biochem. 2010;48:621-626.

Boopathy NS, Kathiresan K. Anticancer drugs from marine flora: An overview. J Oncol. 2010;2010: ID 214186.

Ganesh G, Abhishek T, Saurabh M, Sarada NC. Cytotoxic and apoptosis induction potential of Mimusops elengi L. in human cervical cancer (SiHa) cell line. J King Saud Univ Sci. 2014;26:333-337.

Weli AM, AL-Hinai JRK, Al-Mjrafi JMA, Alnaaimi JRS, Hossain MA, Saeed S, et al. Effect of different polarities leaves crude extracts of Omani Juniperus excelsa on antioxidant, antimicrobial and cytotoxic activities and their biochemical screening. Asian Pac J Reprod. 2014;3:218-223.

Nobili S, Lippi D, Witort E, Donnini M, Bausi L, Mini E, et al. Natural compounds for cancer treatment and prevention. Pharmacol Res. 2009;59:365-378.

Kuppusamy P, Yusoff MM, Maniam GP, Govindan N. A case study regulation and functional mechanisms of cancer cells and control its activity using plants and their derivatives. J Pharm Res. 2013;6:884-892.

Anter J, Fernández-bedmar Z, Villatoro-pulido M, Demyda-peyras S, Moreno-millán M, Alonso-moraga Á, et al. A pilot study on the DNA-protective, cytotoxic, and apoptosis-inducing properties of olive-leaf extracts. Mutat Res. 2011;723:165-170.

Portt L, Norman G, Clapp C, Greenwood M, Greenwood MT. Anti-apoptosis and cell survival: a review. Biochim Biophys Acta. 2011;1813:238-259.

Sun SY, Hail N, Lotan RJ. Apoptosis as a novel target for cancer chemoprevention. J Natl Cancer Inst. 2004;4:662-672.

Thangam R, Sathuvan M, Poongodi A, Suresh V, Pazhanichamy K, Sivasubramanian S, et al. Activation of intrinsic apoptotic signaling pathway in cancer cells by Cymbopogon citratus polysaccharide fractions. Carbohydr Polym. 2014;107:138-150.

Felipe KB, Kviecinski MR, da Silva FO, Bücker NF, Farias MS, Porfirio Will Castro LSE, et al. Inhibition of tumor proliferation associated with cell cycle arrest caused by extract and fraction from Casearia sylvestris (Salicaceae). J Ethnopharmacol. 2014;155:1492-1499.

Shojaee S, Parhiz H, Eshaghi A, Emami SA, Asili J, Mosaffa F. In vitro protective effects of Scutellaria litwinowii root extract against H2O2-induced DNA damage and cytotoxicity. J Complement Integr Med. 2014;11:121-127.

Ahmadi F, Mojarrab M, Ghazi-Khansari M, Hosseinzadeh L. A semipolar fraction of petroleum ether extract of Artemisia aucheri induces apoptosis and enhances the apoptotic response to doxorubicin in human neuroblastoma SKNMC cell line. Res Pharm Sci. 2015;10:335-344.

Tayarani-Najaran Z, Mousavi SH, Asili J, Emami SA. Growth-inhibitory effect of Scutellaria lindbergii in human cancer cell lines. Food Chem Toxicol. 2010;48:599–604.

Tayarani-Najaran Z, Amiri A, Karimi G, Emami SA, Asili J, Mousavi SH. Comparative studies of cytotoxic and apoptotic properties of different extracts and the essential oil of Lavandula angustifolia on malignant and normal cells. Nutr Cancer. 2014;66:424-434.

Tayarani-Najaran Z, Asili J, Aioubi E, Emami SA. Growth inhibition and apoptosis induction of Salvia chloroleuca on MCF-7 breast cancer cell line. Iran J Pharm Res. 2013;12:789-799.

Tayarani-Najaran Z, Mousavi SH, Tajfard F, Asili J, Soltani S, Hatamipour M, et al. Cytotoxic and apoptogenic properties of three isolated diterpenoids from Salvia chorassanica through bioassay-guided fractionation. Food Chem Toxicol. 2013;57: 346-351.

Parsaee H, Asili J, Mousavi SH, Soofi H, Emami SA, Tayarani-Najaran Z. Apoptosis induction of Salvia chorassanica root extract on human cervical cancer cell line. Iran J Pharm Res. 2013;12:75-83.

Kharazian N. Flavonoid constituents in some of endemic Salvia L. (Lamiaceae) species in Iran. Res Pharm Sci. 2012;7:S752.

Boozari M, Mohammadi A, Asilia A, Emami SA, Tayarani-Najaran Z. Growth inhibition and apoptosis induction by Scutellaria pinnatifida A. Ham. on HL-60 and K562 leukemic cell lines. Environ Toxicol Pharmacol. 2015; 39:307-312.

O'Brien J, Wilson I, Orton T, Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem. 2000;267: 5421-5426.

Cury-Boaventura MF, Pomp C, Curi R. Comparative toxicity of oleic acid and linoleic acid on Jurkat cells. Clin Nutr. 2004;23:721-732.

Angelova S, Gospodinova Z, Krasteva M, Antov G, Lozanov V, Bozhanov S, et al. Antitumor activity of Bulgarian herb Tribulus terrestris L. on human breast cancer cells. J BioSci Biotech. 2013;2:25-32.

Chen P, Shih Y, Huang H, Cheng H. Diosgenin, a steroidal saponin, inhibits migration and invasion of human prostate cancer PC-3 cells by reducing matrix metalloproteinases expression. PLoS One. 2011;6:e20164.

Mousavi Sh, Tayrani-najaran Z, Hersey P. Apoptosis: from signalling pathways to therapeutic tools. Iran J Basic Med Sci. 2008;11:121-142.

Xavier CPR, Lima CL, Ferreira MF, Pereira-wilson C. Salvia fruticosa, Salvia officinalis, and rosmarinic acid induce apoptosis and inhibit proliferation of human colorectal cell lines : The role in MAPK/ERK pathway. Nutr Cancer. 2009;61: 564-571.

Tundis R, Loizzo MR, Menichini F, Bonesi M, Colica C, Menichini F. In vitro cytotoxic activity of extracts and isolated constituents of Salvia leriifolia Benth. against a panel of human cancer cell lines. Chem Biodivers. 2011;8:1152-1162.

Zare Shahneh F, Valiyari S, Baradaran B, Abdolalizadeh J, Bandehagh A, Azadmehr A, et al. Inhibitory and cytotoxic activities of Salvia officinalis L. extract on human lymphoma and leukemia cells by induction of apoptosis. Adv Pharm Bull. 2013;3:51-55.


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