Amplification and cloning of Taq DNA polymerase gene from Thermus Aquaticus strain YT-1

H. Mir Mohammad Sadeghi, M. Rabbani, F. Moazen


DNA amplification using Taq DNA polymerase is one of the most widely used techniques in molecular biology and biotechnology. The aim of this study was to amplify the gene of this enzyme from a thermophilic bacteria called Thermus aqauticus and clone it into a vector for future use. Using specific primers the cDNA of Taq DNA polymerase was amplified and ligated into the cloning vector pTZ57R using TA cloning technique. The recombinant plasmids were identified using  restriction enzyme digestion. The presence of the Taq DNA polymerase gene was confirmed by DNA sequencing. In conclusion, Taq DNA polymerase gene has been cloned in our laboratory and can be used for the production of large quantities of this enzyme.


Cloning, Taq DNA polymerase, Thermus aquaticus

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons LicenseThis work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.